Proton NMR assignments and solution conformation of RANTES, a chemokine of the C-C type

Biochemistry
N J SkeltonT J Schall

Abstract

1H NMR has been used to investigate the structural properties of RANTES, a protein from the C-C branch of the chemotactic cytokine family that has a strong chemoattractive effect on monocytes, lymphocytes, and eosinophils. Titration of pH from 5.0 to 2.5 indicates that RANTES is extensively aggregated in solution above pH 4.0. At pH 3.7 the protein is mostly dimeric, although this species does dissociate to the monomer with a Kd of 35 microM. NMR data have been acquired and resonance assignments made for the dimeric species. Structures of the dimer have been generated by distance geometry and simulated annealing calculations that utilized 1956 intramolecular distance restraints, 120 intermolecular distance restraints, 164 dihedral angle restraints, and 68 restraints enforcing 34 hydrogen bonds (17.0 restraints per residue). The structure is well-defined (average root mean square deviation from the average structure of 0.38 +/- 0.06 and 0.53 +/- 0.12 A for backbone heavy atoms of residues 4-66 of the monomer and dimer, respectively). Each monomer consists of a C-terminal alpha-helix packing against a three-stranded antiparallel beta-sheet and two short N-terminal beta-strands; dimerization occurs between the N-terminal regions o...Continue Reading

Citations

Mar 1, 1997·Protein Science : a Publication of the Protein Society·S R LeongC A Hébert
Apr 5, 2001·Trends in Immunology·V Appay, S L Rowland-Jones
Jan 5, 2011·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Paolo LussoVincenzo Pavone
Dec 14, 1999·Protein Science : a Publication of the Protein Society·A C LiwangP J Liwang
Jan 1, 1997·Annual Review of Immunology·M BaggioliniB Moser
Feb 13, 2007·Annual Review of Immunology·Samantha J AllenTracy M Handel
Jul 13, 2011·International Journal of Molecular Sciences·Joshua J ZiarekBrian F Volkman
May 1, 1997·European Journal of Immunology·M C SzaboK B Bacon
Jan 1, 1995·Critical Reviews in Biochemistry and Molecular Biology·A M Gronenborn, G M Clore
Nov 28, 2012·Immunology Letters·Mehdi Houimel, Luca Mazzucchelli
May 21, 2009·Biochimica Et Biophysica Acta·Angelika RekAndreas J Kungl
Feb 5, 2008·Vaccine·Luca VangelistaPaolo Lusso
Nov 15, 2006·Journal of Molecular Biology·Luminita DumaStephan Grzesiek
Mar 21, 2015·Scientific Reports·Inger ØynebråtenGuttorm Haraldsen
Mar 14, 2013·The FEBS Journal·Einat SchnurJacob Anglister
Aug 24, 2005·Biochemical and Biophysical Research Communications·Mayuko MoriZiwei Huang
Nov 1, 2005·Biochemical and Biophysical Research Communications·Hongjun JinPatricia J LiWang
Jun 1, 2006·Biopolymers·Nannette Y YountMichael R Yeaman
Nov 16, 2010·Journal of Structural Biology·Xu WangJames H Prestegard
Jun 23, 2015·Frontiers in Immunology·Tracy Marie Handel
Apr 5, 2001·Clinical Immunology : the Official Journal of the Clinical Immunology Society·D D PatelM S Krangel
Feb 25, 2015·Immunology and Cell Biology·Irina KufarevaTracy M Handel
May 10, 2003·Structure·G Jawahar SwaminathanK Ravi Acharya

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.