PMID: 41713Oct 15, 1979

Proton-nuclear-magnetic-resonance/pH-titration studies of the histidines of pancreatic phospholipase A2

European Journal of Biochemistry
A AguiarR J Williams

Abstract

The study by means of 1H nuclear magnetic resonance (NMR) of the histidines of phospholipase A2 isolated from porcine, bovine and equine pancreas is reported. Assignment of the histidine resonances was achieved by comparison of different enzymes and the use of paramagnetic probes. pH titration curves for various histidyl resonances were obtained and compared in the presence and absence of calcium. Calcium is shown to lower the pKa of the active site histidine. The NMR results are compared with the known X-ray three-dimensional structure for the bovine enzyme.

References

Dec 18, 1975·Biochimica Et Biophysica Acta·F M Van Wezel, G de Haas
Jan 2, 1978·European Journal of Biochemistry·E A FleerG de Haas
Sep 5, 1978·Journal of Molecular Biology·B W DijkstraP J Vandermaelen
Jun 24, 1977·Biochimica Et Biophysica Acta·W C PuijkG de Haas
Jan 1, 1974·Methods in Enzymology·W NieuwenhuizenG de Haas
Mar 26, 1974·Biochemistry·W A PietersonG de Haas
Mar 26, 1974·Biochemistry·J J VolwerkG de Haas
Apr 24, 1968·Biochimica Et Biophysica Acta·G de HaasL L van Deenen
Apr 24, 1968·Biochimica Et Biophysica Acta·G de HaasL L van Deenen
Oct 22, 1963·Biochimica Et Biophysica Acta· VAN DEENENL, G de Haas

Citations

Nov 1, 1994·Protein Science : a Publication of the Protein Society·A KumarM Sundaralingam
May 11, 2002·Behaviour Research and Therapy·D Veale
Jan 27, 2015·Journal of Chromatography. a·Katharina M H LangJürgen Hubbuch
May 10, 1983·Biochemistry·G M Donné-Op den KelderM R Egmond
Dec 4, 1984·Biochemistry·G J van ScharrenburgA J Slotboom
Feb 1, 1980·European Journal of Biochemistry·G R Moore, R J Williams

Related Concepts

Bos indicus
Histidine
Hydrogen-Ion Concentration
In Vivo NMR Spectroscopy
Pancreas
Phospholipase
Phospholipases A
Protein Conformation
Phospholipases A2

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