Proton production and consumption pathways in yeast metabolism. A chemostat culture analysis

Yeast
J I CastrilloU Ugalde

Abstract

In this investigation, a method for the accurate quantitative determination of net proton production or consumption in biological cultures has been devised. Cells are cultured under constant pH conditions. The specific rate of proton production or consumption by the culture (qH+, mmol h-1 per g biomass) is proportional to the mmol of base or acid required to maintain constant pH per unit time, and this equivalence is independent of the buffering capacity of the culture medium. The above method has been applied to chemostat cultures of Candida utilis growing on glucose or glycerol as carbon source, and different nitrogen sources. The results indicate that the nitrogen assimilation pathway alone determines the value of qH+, and a fixed stoichiometric relationship between nitrogen uptake rate qN (meq h-1 per g biomass) and qH+ has been found for each nitrogen source employed. Thus, qH+/qN values of +1, 0 and -1 were found for ammonium ions, urea and nitrate respectively. Under oxidative metabolism, the contribution of carbon catabolism to the value of qH+ was undetectable. Sine qN may be related to growth and production of type 1 compounds in fermentation processes, the parameter qH+ was incorporated into a model of growth and ene...Continue Reading

References

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Citations

Apr 21, 2012·International Journal of Microbiology·Vipul Gohel, Gang Duan
Jan 22, 2010·BMC Biotechnology·Karthikeyan Srinivasan, Radhakrishnan Mahadevan
Oct 6, 2009·FEMS Microbiology Letters·Ana Belén Rodríguez-UrraUnai Ugalde
Jul 23, 1999·Biotechnology and Bioengineering·S A Siano
Apr 1, 1999·Biotechnology and Bioengineering·A VicenteU Ugalde
Sep 21, 2007·Fungal Genetics and Biology : FG & B·Miguel A Moreno-MateosTahía Benítez
Feb 4, 2010·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·T TammeA Kiis
Aug 30, 2007·Bioprocess and Biosystems Engineering·Heléne Sundström, Sven-Olof Enfors
Jul 11, 2020·Biotechnology Letters·Mayara C S BarcelosGustavo Molina

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