PMID: 11334783May 4, 2001Paper

Proton transfer in Azotobacter vinelandii ferredoxin I: entatic Lys84 operates as elastic counterbalance for the proton-carrying Asp15

Biochimica Et Biophysica Acta
D A Cherepanov, A Y Mulkidjanian

Abstract

In ferredoxin I from Azotobacter vinelandii, the reduction of a [3Fe-4S] iron-sulphur cluster is coupled with the protonation of the mu2S sulphur atom that is approx. 6 A away from the protein boundary. The recent study of the site-specific mutants of ferredoxin I led to the conclusion that a particular surface aspartic residue (Asp15) is solely responsible for the proton transfer to the mu2S atom by 'rapid penetrative excursions' (K. Chen, J. Hirst, R. Camba, C.A. Bonagura, C.D. Stout, B.K. Burgess, F.A. Armstrong, Nature 405 (2000) 814-817). In the same paper it has been reported that the replacement of Asp15 by glutamate led to the blockage of the enzyme, although glutamate, with its longer and more flexible side chain, should apparently do even better as a mobile proton carrier than aspartate. We tackled this puzzling incompetence of Glu15 by molecular dynamics simulations. It was revealed that the conformational alterations of Asp15 are energetically balanced by the straining of the nearby Lys84 side chain in wild-type ferredoxin I but not in the Asp15-->Glu mutant. Lys84 in ferredoxin I of A. vinelandii seems to represent the first case where the strained (entatic) conformation of a particular amino acid side chain could ...Continue Reading

References

Apr 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·M HolzH G Khorana
Feb 1, 1968·Proceedings of the National Academy of Sciences of the United States of America·B L Vallee, R J Williams
Sep 18, 1997·Proceedings of the National Academy of Sciences of the United States of America·M L VerkhovskayaM Wikström
Sep 1, 1999·Proceedings of the National Academy of Sciences of the United States of America·A R CroftsE A Berry
Nov 11, 1999·Proceedings of the National Academy of Sciences of the United States of America·O A GoptaA Y Mulkidjanian

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Citations

Jan 28, 2003·Current Opinion in Chemical Biology·Lucia Banci
Dec 25, 2012·Coordination Chemistry Reviews·Daniel J Kosman
Dec 12, 2007·Journal of Computational Chemistry·Sven LammersMarkus Meuwly
Dec 10, 2016·Journal of the American Chemical Society·Abhishek SingharoyKlaus Schulten
May 3, 2011·The Journal of Biological Chemistry·Stephan LutzMarkus Meuwly

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