PMID: 30478Oct 11, 1978

Proton-translocating cytochrome c oxidase in artificial phospholipid vesicles

Biochimica Et Biophysica Acta
K Krab, M Wikström

Abstract

The proton translocating properties of cytochrome c oxidase have been studied in artificial phospholipid vesicles into the membranes of which the isolated and purified enzyme was incorporated. Initiation of oxidation of ferrocytochrome c by addition of the cytochrome, or by addition of oxygen to an anaerobic vesicle suspension, leads to ejection of H+ from the vesicles provided that charge compensation is permitted by the presence of valinomycin and K+. Proton ejection is not observed if the membranes have been specifically rendered permeable to protons. The proton ejection is the result of true translocation of H+ across the membrane as indicated by its dependence on the intravesicular buffering power relative to the number of particles (electrons and protons) transferred by the system, and since it can be shown not to be due to a net formation of acid in the system. Comparison of the initial rates of proton ejection and oxidation of cytochrome c yields a H+/e- quotient close to 1.0 both in cytochrome c and oxygen pulse experiments. An approach towards the same stoichiometry is found by comparison of the extents of proton ejection and electron transfer under appropriate experimental conditions. It is concluded that cytochrome ...Continue Reading

References

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Citations

Apr 1, 1984·Journal of Bioenergetics and Biomembranes·J A Freedman, S H Chan
Dec 1, 1981·Journal of Bioenergetics and Biomembranes·R P CaseyA Azzi
Feb 1, 1986·Journal of Bioenergetics and Biomembranes·B Kadenbach
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Oct 3, 2002·Protein Expression and Purification·Xuan-Thao NguyenL J Prochaska
Feb 1, 1981·Quarterly Reviews of Biophysics·M MontalH Schindler
Apr 25, 2012·Proceedings of the National Academy of Sciences of the United States of America·Virve RauhamäkiM Wikström
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Oct 24, 2007·Antioxidants & Redox Signaling·Ilya Belevich, Michael I Verkhovsky
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May 17, 2000·Biochimica Et Biophysica Acta·Dmitry Zaslavsky, R B Gennis
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Related Concepts

Aerobiosis
Metazoa
Bos indicus
Cytochrome c Oxidase Subunit VIa
Hydrogen-Ion Concentration
Membranes, Artificial
Mitochondria, Heart
Pandavir
Osmolality
Oxidation-Reduction

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