Proton translocation coupled to trimethylamine N-oxide reduction in anaerobically grown Escherichia coli.

Journal of Bacteriology
M TakagiM Ishimoto

Abstract

Proton translocation coupled to trimethylamine N-oxide reduction was studied in Escherichia coli grown anaerobically in the presence of trimethylamine N-oxide. Rapid acidification of the medium was observed when trimethylamine N-oxide was added to anaerobic cell suspensions of E. coli K-10. Acidification was sensitive to the proton conductor 3,5-di-tert-butyl-4-hydroxybenzylidenemalononitrile (SF6847). No pH change was shown in a strain deficient in trimethylamine N-oxide reductase activity. The apparent H+/trimethylamine N-oxide ratio in cells oxidizing endogenous substrates was 3 to 4 g-ions of H+ translocated per mol of trimethylamine N-oxide added. The addition of trimethylamine N-oxide and formate to ethylenediaminetetraacetic acid-treated cell suspension caused fluorescence quenching of 3,3'-dipropylthiacarbocyanine [diS-C3-(5)], indicating the generation of membrane potential. These results indicate that the reduction of trimethylamine N-oxide in E. coli is catalyzed by an anaerobic electron transfer system, resulting in formation of a proton motive force. Trimethylamine N-oxide reductase activity and proton extrusion were also examined in chlorate-resistant mutants. Reduction of trimethylamine N-oxide occurred in chlC, ...Continue Reading

References

Dec 1, 1975·The Biochemical Journal·P B GarlandB A Haddock
Aug 29, 1978·Biochemical and Biophysical Research Communications·K Miki, T H Wilson
Jun 1, 1979·Journal of General Microbiology·A R StrømH Larsen
Dec 1, 1974·Canadian Journal of Microbiology·K E Kim, G W Chang
Apr 1, 1973·Journal of Biochemistry·M Sagai, M Ishimoto
Apr 1, 1968·Journal of Bacteriology·M K Showe, J A DeMoss
Jan 1, 1968·Molecular & General Genetics : MGG·W A Venables, J R Guest
Jun 6, 1969·Biochemical and Biophysical Research Communications·J PuigJ Gendre
Mar 1, 1971·Journal of Bacteriology·R L Lester, J A DeMoss
Jun 1, 1980·Journal of Bacteriology·M T MadiganH Gest
Mar 1, 1980·Microbiological Reviews·B J Bachmann, K B Low

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Citations

Oct 21, 2016·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Diede FennemaElizabeth A Shephard
Mar 1, 1994·Molecular Microbiology·V MéjeanM C Pascal
Jul 1, 1989·Journal of Bacteriology·P A Cotter, R P Gunsalus
Sep 1, 1984·Microbiological Reviews·W J Ingledew, R K Poole
Oct 31, 1983·Biochimica Et Biophysica Acta·P D Bragg, N R Hackett
Jul 1, 1986·Journal of Bacteriology·J W Winkelman, D P Clark
Apr 1, 2007·EcoSal Plus·Victor W T Cheng, Joel H Weiner
Apr 29, 1986·Biochemical and Biophysical Research Communications·M Hinojosa-LeonM Chippaux

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