PMID: 6290474Nov 10, 1982

Proton transport by cytochrome c oxidase from the thermophilic bacterium PS3 reconstituted in liposomes

The Journal of Biological Chemistry
N Sone, P C Hinkle

Abstract

Cytochrome oxidase from the thermophilic bacterium PS3 which contains three types of polypeptide subunits are reconstituted into liposomes by a freeze-thaw technique. The reconstituted enzyme caused acidification of the medium during cytochrome c oxidation with a stoichiometry of up to 0.8 H+/e. Uptake of K+ ions in the presence of valinomycin occurred with a stoichiometry between 1.5 and 2 K+/e. Dicyclohexylcarbodiimide inhibited the acidification and decreased the stoichiometry of K+ ion uptake to about 1 K+/e. This bacterial oxidase thus appears to be a proton pump with properties similar to the mitochondrial enzyme.

Related Concepts

Cytochrome c Oxidase Subunit VIa
Dicyclohexylcarbodiimide
Hydrogen-Ion Concentration
Niosomes
Potassium

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