Protonation studies and multivariate curve resolution on oligodeoxynucleotides carrying the mutagenic base 2-aminopurine

Biophysical Journal
Raimundo GargalloR Eritja

Abstract

2-Aminopurine (P) is a mutagen causing A.T to G.C transitions in prokaryotic systems. To study the base-pairing schemes between P and cytosine (C) or thymine (T), two self-complementary dodecamers containing P paired with either C or T were synthesized, and their protonation equilibria were studied by acid-base titrations and melting experiments. The mismatches were incorporated into the self-complementary sequence d(CGCPCCGGXGCG), where X was C or T. Spectroscopic data obtained from molecular absorption, circular dichroism (CD), and molecular fluorescence spectroscopy were analyzed by a factor-analysis-based method, multivariate curve resolution based on the alternating least squares optimization procedure (MCR-ALS). This procedure allows determination of the number of acid-base species or conformations present in an acid-base or melting experiment and the resolution of the concentration profiles and pure spectra for each of them. Acid-base experiments have shown that at pH 7, 150 mM ionic strength, and 37 degrees C, both C and P are deprotonated. At pH near 4, the majority of species shows C protonated and P deprotonated. Finally, at pH values near 3, the majority of species shows both protonated C and P. These results are in...Continue Reading

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Citations

Mar 23, 2004·Analytical Biochemistry·Joaquim JaumotRaimundo Gargallo
Nov 25, 2003·Journal of Chemical Information and Computer Sciences·L DuponchelJ P Huvenne
Dec 8, 2020·Dalton Transactions : an International Journal of Inorganic Chemistry·Francesco TavaniPaola D'Angelo
Jul 1, 2020·Inorganic Chemistry·Francesco TavaniPaola D'Angelo
Jun 8, 2014·International Journal of Biological Macromolecules·Mohammad-Bagher GholivandHector C Goicoechea
Apr 15, 2004·Chemical Reviews·Carlos E Crespo-HernándezBern Kohler

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