Protoplast isolation and regeneration in Streptomyces clavuligerus.

Journal of General Microbiology
G T IllingJ F Peberdy

Abstract

The regeneration of streptomycete protoplasts is a major step following genetic manipulations such as fusion and DNA-mediated transformation. Reports of studies on the regeneration of protoplasts from Streptomyces clavuligerus are limited and for this reason the experiments described in this paper were carried out. An investigation of protoplast formation and cytology was made to gain further insight into the loss of protoplast viability in osmotically stabilized support media. Protoplasts with the highest regeneration frequency were isolated from mycelium, grown in a two-stage culture system (without glycine), using lysozyme dissolved in a sucrose osmoticum containing 1% bovine serum albumin. The latter promoted improved protoplast viability. A systematic survey was made of the components of regeneration medium R5, previously used for S. clavuligerus, and other potentially advantageous components and conditions, in an attempt to raise the regeneration frequency of the protoplasts. An improved regeneration medium (R6) and protocol which supported higher and more consistent levels of regeneration of S. clavuligerus protoplasts resulted from these experiments. These improved procedures for protoplast isolation and regeneration pr...Continue Reading

Citations

Jun 14, 2008·Applied Biochemistry and Biotechnology·Mervat M A El-GendyK A Shaaban
Jan 1, 1993·Biotechnology Advances·D V GokhaleD N Deobagkar
Apr 1, 1993·Letters in Applied Microbiology·D LyutzkanovaT Todorov
Apr 3, 2016·Applied and Environmental Microbiology·Tina NetzkerAxel A Brakhage
Apr 14, 2017·Bioscience, Biotechnology, and Biochemistry·Tetsuya YabutaniHideo Nakano

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacterial Cell Wall Structure

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.

Bacterial Cell Wall Structure (ASM)

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.