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Protoplasts of Schizosaccharomyces pombe: an improved method for their preparation and the study of their guanine uptake

Journal of General Microbiology

Oct 1, 1975

P HoussetJ Schwencke

Abstract

A new method is described for the efficient conversion of Schizosaccharomyces pombe cells into protoplasts. The following parameters of guanine uptake determined in whole cells were unchanged in protoplasts: Km value, requirement for an energy source, sensitivity to competitive inhibito...read more

Mentioned in this Paper

Hydrogen-Ion Concentration
Hypoxanthines
Polysaccharides
Protoplasts
Saccharomyces
Snails
Azaguanine
Azides
Cell Wall
Cochliobolus

Protoplasts of Schizosaccharomyces pombe: an improved method for their preparation and the study of their guanine uptake

Journal of General Microbiology

Oct 1, 1975

P HoussetJ Schwencke

PMID: 463

DOI: 10.1099/00221287-90-2-260

Abstract

A new method is described for the efficient conversion of Schizosaccharomyces pombe cells into protoplasts. The following parameters of guanine uptake determined in whole cells were unchanged in protoplasts: Km value, requirement for an energy source, sensitivity to competitive inhibito...read more

Mentioned in this Paper

Hydrogen-Ion Concentration
Hypoxanthines
Polysaccharides
Protoplasts
Saccharomyces
Snails
Azaguanine
Azides
Cell Wall
Cochliobolus
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