Oct 1, 1975

Protoplasts of Schizosaccharomyces pombe: an improved method for their preparation and the study of their guanine uptake

Journal of General Microbiology
P HoussetJ Schwencke

Abstract

A new method is described for the efficient conversion of Schizosaccharomyces pombe cells into protoplasts. The following parameters of guanine uptake determined in whole cells were unchanged in protoplasts: Km value, requirement for an energy source, sensitivity to competitive inhibitors, pH optimum, as well as the typical variation of the initial velocity of uptake observed during the growth phase.

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Mentioned in this Paper

Guanine
Hypoxanthines
Azaguanine
Protoplasts
Saccharomyces
Cell Wall
Exoglycosidases
Cochliobolus
Snails
Schizosaccharomyces pombe

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