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Protospacer adjacent motif (PAM)-distal sequences engage CRISPR Cas9 DNA target cleavage

PloS One

Oct 3, 2014

Regina CencicJerry Pelletier

Abstract

The clustered regularly interspaced short palindromic repeat (CRISPR)-associated enzyme Cas9 is an RNA-guided nuclease that has been widely adapted for genome editing in eukaryotic cells. However, the in vivo target specificity of Cas9 is poorly understood and most studies rely on in si...read more

Mentioned in this Paper

TP53 gene
Study
In Vivo
Non-Homologous DNA End-Joining
Flow Cytometry
Exploration With a Probe
Polymerase
Heparin
DNA Probes
Exons
3
240
Paper Details
References
  • References33
  • Citations36
1234
  • References33
  • Citations36
1234

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Genome-wide binding of the CRISPR endonuclease Cas9 in mammalian cells

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Protospacer adjacent motif (PAM)-distal sequences engage CRISPR Cas9 DNA target cleavage

PloS One

Oct 3, 2014

Regina CencicJerry Pelletier

PMID: 25275497

DOI: 10.1371/journal.pone.0109213

Abstract

The clustered regularly interspaced short palindromic repeat (CRISPR)-associated enzyme Cas9 is an RNA-guided nuclease that has been widely adapted for genome editing in eukaryotic cells. However, the in vivo target specificity of Cas9 is poorly understood and most studies rely on in si...read more

Mentioned in this Paper

TP53 gene
Study
In Vivo
Non-Homologous DNA End-Joining
Flow Cytometry
Exploration With a Probe
Polymerase
Heparin
DNA Probes
Exons
3
240

Similar Papers Found In These Feeds

p53 Pathway

p53 is a transcription factor that is involved in several cellular processes, including cell cycle arrest, cellular apoptosis, cell death and cell senescence. Mutations in the wild type p53 gene has been implicated in a significant number of cancers. Here is the latest research on p53 pathways.

CRISPR (general)

Clustered regularly interspaced short palindromic repeats (CRISPR) are DNA sequences in the genome that are recognized and cleaved by CRISPR-associated proteins (Cas). CRISPR-Cas system enables the editing of genes to create or correct mutations. Discover the latest research on CRISPR here.

Related Papers

Nature Biotechnology

Genome-wide binding of the CRISPR endonuclease Cas9 in mammalian cells

Nature BiotechnologyApril 23, 2014
Xuebing WuPhillip A Sharp
Nature Biotechnology

Improving CRISPR-Cas nuclease specificity using truncated guide RNAs

Nature BiotechnologyJanuary 28, 2014
Yanfang FuJ Keith Joung
Paper Details
References
  • References33
  • Citations36
1234
  • References33
  • Citations36
1234
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