Prototropism of 1-hydroxypyrene in liposome suspensions: implications towards fluorescence probing of lipid bilayers in alkaline medium

Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology
Usharani Subuddhi, Ashok K Mishra

Abstract

The partitioning efficiency of neutral and anionic prototropic forms of 1-hydroxypyrene in liposome suspensions has been studied. The high partition coefficient value of 1-hydroxypyrene indicates an easy incorporation of the molecule into the lipid bilayer. Detailed pH studies indicate that only the neutral form of 1-hydroxypyrene partitions into the membrane and appreciable spectral changes are observed in the pH range of 9.0-11.5 in Tris-NaOH buffer. However, at pH 11 the spectral changes are maximum. The possibility of using 1-hydroxypyrene as a fluorescent molecular probe for lipid bilayer membranes in alkaline media has been examined, by employing fluorescence intensity and fluorescence anisotropy as probe parameters. The neutral form fluorescence intensity as well as fluorescence anisotropy is sensitive to the changes in the membrane properties and is capable of sensing the phase-transition. This is also capable of monitoring the changes in the membrane due to incorporation of cholesterol and the ethanol-induced interdigitation. The time resolved fluorescence data and the quenching experiments show that 1-hydroxypyrene occupies the water inaccessible interior of the liposome. The high anisotropy value of 1-hydroxypyrene i...Continue Reading

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Citations

Jan 31, 2012·Journal of Agricultural and Food Chemistry·Yumeng NiuWeiguo Shen
Aug 30, 2008·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·T Sanjoy Singh, Sivaprasad Mitra
Oct 1, 2009·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Monalisa MohapatraAshok K Mishra
Sep 15, 2011·Physical Chemistry Chemical Physics : PCCP·Elena CariatiDario Pasini
Nov 17, 2006·Annals of the New York Academy of Sciences·Elena Y RykovaValentin V Vlassov

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