proTRH gene expression by fetal pancreatic islets in culture

Biochemical and Biophysical Research Communications
S Aratan-SpireA H Tashjian

Abstract

The hypothalamic tripeptide, thyrotropin-releasing hormone (TRH), has been detected in neonatal pancreatic tissue and localized by immunocytochemistry in the islets of Langerhans. To determine whether the TRH gene is expressed in islets, we have extracted RNA from cultured rat islets and probed for proTRH mRNA using a [32P]-labeled antisense RNA. Islet proTRH mRNA comigrated with the 1.6 kilobase proTRH mRNA present in the rat hypothalamus. Normalized to total RNA, islets cultured for 7 days contained at least 10 times more proTRH mRNA than day 1 whole pancreas. We conclude that pancreatic TRH is synthesized in situ in the islets of Langerhans. This is the first attempt to characterize and quantify proTRH mRNA using neoformed foetal islets. We propose that quantitative analysis of proTRH mRNA concentrations in this culture system will enable study of the direct regulation of TRH biosynthesis in the pancreas.

References

Oct 13, 1978·Brain Research·A Faivre-BaumanA Tixier-Vidal
Sep 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·E MartinoS Refetoff
Aug 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·G TeitelmanD J Reis
May 1, 1984·Acta Endocrinologica·S Aratan-SpireP Czernichow

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