Prox1 Is a Marker for AII Amacrine Cells in the Mouse Retina

Frontiers in Neuroanatomy
Luis Pérez de Sevilla MüllerNicholas C Brecha

Abstract

The transcription factor Prox1 is expressed in multiple cells in the retina during eye development. This study has focused on neuronal Prox1 expression in the inner nuclear layer (INL) of the adult mouse retina. Prox1 immunostaining was evaluated in vertical retinal sections and whole mount preparations using a specific antibody directed to the C-terminus of Prox1. Strong immunostaining was observed in numerous amacrine cell bodies and in all horizontal cell bodies in the proximal and distal INL, respectively. Some bipolar cells were also weakly immunostained. Prox1-immunoreactive amacrine cells expressed glycine, and they formed 35 ± 3% of all glycinergic amacrine cells. Intracellular Neurobiotin injections into AII amacrine cells showed that all gap junction-coupled AII amacrine cells express Prox1, and no other Prox1-immunostained amacrine cells were in the immediate area surrounding the injected AII amacrine cell. Prox1-immunoreactive amacrine cell bodies were distributed across the retina, with their highest density (3887 ± 160 cells/mm2) in the central retina, 0.5 mm from the optic nerve head, and their lowest density (3133 ± 350 cells/mm2) in the mid-peripheral retina, 2 mm from the optic nerve head. Prox1-immunoreactive...Continue Reading

References

Mar 22, 1978·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·H Wässle, H J Riemann
Nov 8, 1992·The Journal of Comparative Neurology·E StrettoiR F Dacheux
Aug 8, 1991·The Journal of Comparative Neurology·H M Young, D I Vaney
Feb 15, 1991·The Journal of Comparative Neurology·S L Mills, S C Massey
Apr 1, 1991·Physiological Reviews·H Wässle, B B Boycott
Jul 1, 1990·Visual Neuroscience·B PasteelsR Pochet
Dec 2, 1985·Brain Research·R G Pourcho, D J Goebel
Sep 22, 1989·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·D I VaneyH M Young
Jun 22, 1985·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·D I Vaney
Aug 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·N BrechaH Wässle
Oct 4, 1974·Science·H Kolb, E V Famiglietti
May 22, 1995·The Journal of Comparative Neurology·G CasiniN C Brecha
Nov 1, 1993·Mechanisms of Development·G OliverP Gruss
Feb 1, 1994·Trends in Biochemical Sciences·T R Bürglin
Jun 22, 1993·The Journal of Comparative Neurology·H WässleJ Röhrenbeck
Jun 22, 1993·The Journal of Comparative Neurology·M H ChunH Wässle
Jul 1, 1996·Current Eye Research·R G Pourcho
Jan 1, 1996·Visual Neuroscience·U Grünert, H Wässle
Dec 10, 1996·Proceedings of the National Academy of Sciences of the United States of America·E Strettoi, R H Masland
Jun 10, 1998·Neuron·M A MacNeil, R H Masland
Nov 5, 1998·The Journal of Comparative Neurology·N MengerH Wässle
Jul 25, 2000·The Journal of Comparative Neurology·D S Rice, T Curran
Aug 31, 2001·Nature Neuroscience·R H Masland
Oct 10, 2002·The Journal of Comparative Neurology·Helga KolbNicolas Cuenca
Sep 25, 2003·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Tiffany Cook
Dec 4, 2003·The Journal of Comparative Neurology·Silke Haverkamp, Heinz Wässle
Dec 23, 2003·The Journal of Comparative Neurology·Krishna K GhoshHeinz Wässle
Feb 13, 2004·The Journal of Comparative Neurology·Eun-Jin LeeMyung-Hoon Chun
Jul 23, 2004·The Journal of Comparative Neurology·Vincenzo Pignatelli, Enrica Strettoi
Aug 27, 2004·The Journal of Comparative Neurology·Juliette JohnsonDavid R Copenhagen
Nov 13, 2004·Vision Research·Stewart A Bloomfield, Béla Völgyi
Dec 24, 2005·The Journal of Comparative Neurology·Eun-Jin LeeNorberto M Grzywacz
Oct 25, 2006·Journal of Biomedical Science·Wen Shen, Zheng Jiang
Dec 22, 2006·The Journal of Comparative Neurology·Andy J FischerWilliam K Stell
Sep 14, 2007·The Journal of Comparative Neurology·Luis Pérez De Sevilla MüllerReto Weiler
Oct 11, 2007·The Journal of Comparative Neurology·Patrick W KeeleyBenjamin E Reese

❮ Previous
Next ❯

Citations

Aug 30, 2018·PloS One·Nicolas D LutzLeo Peichl
Oct 27, 2017·The Journal of Comparative Neurology·Patrick W Keeley, Benjamin E Reese
Feb 7, 2020·The Journal of Comparative Neurology·Patrick W KeeleyBenjamin E Reese
Nov 3, 2020·The Journal of Comparative Neurology·Michael J CamerinoPeter G Fuerst
Jan 1, 2021·Toxicologic Pathology·Anantharaman MuthuswamyOlulanu H Aina
May 15, 2021·Visual Neuroscience·Seema BanerjeeFeng Pan
Jun 8, 2021·Frontiers in Neuroanatomy·Ashley M ChenLuis Pérez de Sevilla Müller

❮ Previous
Next ❯

Methods Mentioned

BETA
transgenic

Software Mentioned

GraphPad Prism
Zeiss LSM 510
GraphPad
Adobe Photoshop CS2
ImageJ

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.