PMID: 374620Jan 1, 1979Paper

Proximity of lectin receptors on the cell surface measured by fluorescence energy transfer in a flow system

The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
S S ChanT M Jovin

Abstract

Molecules of the lectin concanavalin A have been labeled separately with the fluorescein and rhodamine chromophores and jointly bound to the surface of transformed Friend erythroleukemia cells. The two dyes constitute an ideal donor-acceptor pair for fluorescence resonance energy transfer thereby permitting the determination of the proximity relationships between bound ligand molecules and the corresponding surface receptors. The transfer efficiency at saturation (about 57%) was measured in a multiparameter flow system using laser excitation at 488 nm and detection of fluorescein and rhodamine emission intensities as well as the emission anisotropy of the rhodamine fluorescence for each cell. The degree of energy transfer was estimated from the quenching of donor emission, the sensitization of acceptor emission, and the depolarization of acceptor fluorescence. The system has been modeled according to a formalism developed by Gennis and Cantor (Biochemistry 11: 2509, 1972). We estimate the separation between the surfaces of bound lectin molecules at saturation to be 0-40 A, a range possibly characteristic for micropatches induced by ligand binding.

Citations

May 11, 2000·Cytometry·C L AsburyG van den Engh
Jul 3, 2004·European Biophysics Journal : EBJ·Andrew H A ClaytonEdouard C Nice
Oct 1, 1987·Immunology Letters·L TrónS Damjanovich
Jun 1, 1993·Journal of Photochemistry and Photobiology. B, Biology·J MatkoS Damjanovich
Nov 1, 1988·Quarterly Reviews of Biophysics·J MatkóS Damjanovich
Aug 2, 2006·Molecular BioSystems·Eric Trinquet, Gérard Mathis
Nov 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·R H AustinT M Jovin
Oct 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·S DamjanovichT M Jovin
Feb 14, 1995·Proceedings of the National Academy of Sciences of the United States of America·S DamjanovichT M Jovin
Aug 2, 2003·Biology of Reproduction·Deborah A Roess, Steven M L Smith
Oct 22, 2010·Journal of Biomaterials Science. Polymer Edition·Uta ReibetanzBjörn Neu
Jul 8, 1997·Proceedings of the National Academy of Sciences of the United States of America·A JeneiS Damjanovich
Jul 2, 2003·Proceedings of the National Academy of Sciences of the United States of America·G VerebS Damjanovich
Dec 29, 2004·Leukemia & Lymphoma·Meir Cohen-KashiMordechai Deutsch
Mar 31, 2015·International Journal of Molecular Sciences·Dilip ShresthaJános Szöllősi
Dec 20, 2014·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Jakub NedbalSimon M Ameer-Beg
Dec 20, 2002·Acta Physiologica Hungarica·S DamjanovichL Bene
Jan 1, 1988·Cytometry. Supplement : the Journal of the Society for Analytical Cytology·R A Hoffman
Sep 1, 1980·Cytometry·D J Arndt-JovinT M Jovin
Oct 12, 2010·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Ákos I FábiánAttila Jenei
Aug 22, 2003·The Journal of Biological Chemistry·Mary Hunzicker-DunnDeborah A Roess
Oct 22, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Samantha E KerryJeffrey A Frelinger
Sep 21, 1992·Biochimica Et Biophysica Acta·M Esmann
Sep 15, 1982·Analytical Biochemistry·H S Kruth

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.