ProxyPhos sensors for the detection of negatively charged membranes

The Analyst
B I Murcar-EvansP T Gunning

Abstract

Membrane-embedded negatively charged phospholipids (MENCP) can be used as biomarkers for a range of biological processes, including early detection of apoptosis in animal cells, drug-induced phospholipidosis, and selective detection of bacterial over animal cells. Currently, several technologies for the detection of apoptosis and bacterial cells are based on the recognition of MENCPs, including the AnnexinV stain and PSVue™ probes. As probes, these technologies have limitations, the most significant of which is the need for washing the unbound probe away to achieve optimal signal. In contrast, a turn-on chemosensor selective for MENCP would address this shortcoming, and allow for a more rapid protocol for the detection of apoptosis, bacteria and for other relevant applications. In this work, the aim was to explore whether ProxyPhos chemosensors, previously reported by our group for the detection of proximally phosphorylated peptides and proteins, could be re-purposed for the detection of MENCPs. Six lead ProxyPhos sensors were screened against synthetic vesicles containing biologically relevant negatively charged phospholipids including phosphatidic acid (PA), phosphatidylglycerol (PG), cardiolipin (CL) and phosphatidylserine (...Continue Reading

References

Jun 1, 1986·Chemistry and Physics of Lipids·P R CullisC P Tilcock
Jan 1, 1994·Annual Review of Pharmacology and Toxicology·A Y Chen, L F Liu
Dec 13, 1996·Annals of the New York Academy of Sciences·F TraganosZ Darzynkiewicz
Oct 29, 1999·European Journal of Biochemistry·K Athenstaedt, G Daum
Jun 9, 2001·Methods in Cell Biology·P WilliamsonR A Schlegel
Mar 28, 2002·Physiological Reviews·Volker Gerke, Stephen E Moss
Apr 16, 2002·Molecular and Cellular Biochemistry·Junor A Barnes, Aldrin V Gomes
May 30, 2002·Journal of the American Chemical Society·Akio OjidaItaru Hamachi
Nov 1, 2002·Journal of Clinical Microbiology·Sven KlaschikFrank Stuber
May 26, 2005·Bioorganic & Medicinal Chemistry·Roger G Hanshaw, Bradley D Smith
Apr 4, 2006·Chemical Communications : Chem Comm·W Matthew LeevyBradley D Smith
Apr 21, 2006·Biochimica Et Biophysica Acta·Catherine L Stace, Nicholas T Ktistakis
Jun 16, 2006·Expert Opinion on Drug Safety·Mark J ReasorRoger G Ulrich
Sep 19, 2006·FEBS Letters·Nora Anderson, Jürgen Borlak
Dec 13, 2006·Toxicology and Applied Pharmacology·Elizabeth Tengstrand BaronasFrank Y Hsieh
Dec 21, 2006·Journal of the American Chemical Society·W Matthew LeevyBradley D Smith
Jun 15, 2007·Toxicologic Pathology·Susan Elmore
Jan 25, 2008·Nature Reviews. Molecular Cell Biology·Gerrit van MeerGerald W Feigenson
Apr 1, 2008·Biochimie·Wei ZhaoMikko Karttunen
Sep 30, 2008·Biochimica Et Biophysica Acta·Richard M Epand, Raquel F Epand
Sep 29, 2009·Critical Reviews in Biochemistry and Molecular Biology·Bengt Fadeel, Ding Xue
Dec 25, 2009·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ricardo A ChaurioUdo S Gaipl
Mar 3, 2010·Annual Review of Biophysics·Peter A Leventis, Sergio Grinstein
Jan 12, 2011·The Journal of Cell Biology·Christof OsmanThomas Langer
Jan 11, 2013·Physiological Reviews·Michal Bohdanowicz, Sergio Grinstein
Sep 1, 2015·Chemistry and Physics of Lipids·Daniel Lopez
Jan 8, 2013·Journal of Chemical Theory and Computation·Thomas LemminMatteo Dal Peraro
Jun 16, 2016·Chemical Communications : Chem Comm·Douglas R RiceBradley D Smith

❮ Previous
Next ❯

Citations

Mar 13, 2019·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Tia S JarvisBradley D Smith
May 6, 2021·Organic & Biomolecular Chemistry·Sarah R HerschedeNathalie Busschaert
Feb 7, 2020·ACS Applied Materials & Interfaces·Bing WanYunlei Zhang

❮ Previous
Next ❯

Methods Mentioned

BETA
flow cytometry
Fluorescence
FCS
flow cytometery

Software Mentioned

ProxyPhos

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis