PrPSc accumulation in fetal cotyledons of scrapie-resistant lambs is influenced by fetus location in the uterus

The Journal of General Virology
Janet AlversonTimothy V Baszler

Abstract

Placentae from scrapie-infected ewes have been shown to accumulate PrPSc when the genotype of the fetus is of a susceptible genotype (VRQ/VRQ, ARQ/VRQ or ARQ/ARQ). Cotyledons from fetuses of genotypes ARR/ARR, ARQ/ARR and ARQ/VRR have previously been shown to be resistant to PrPSc accumulation. By using ewes from a naturally infected scrapie flock, cotyledons from fetuses of multiple births of different genotypes were examined. PrPSc was detected in fetal cotyledons of genotype ARQ/ARQ, but not in cotyledons from their dizygotic twin of genotype ARQ/ARR. This confirms earlier reports of single fetuses of these genotypes, but is the first description of such a finding in twin fetuses, one of each genotype. It is also demonstrated that cotyledons from sibling fetuses of genotypes ARQ/VRQ and ARQ/ARQ have different patterns of PrPSc accumulation depending on whether the dam is of genotype ARQ/ARQ or ARQ/VRQ. Lastly, it is shown that cotyledons from fetuses with resistant genotypes are weakly positive for PrPSc when they have shared the same pregnant uterine horn with a fetus of a susceptible genotype with cotyledons positive for the detection of PrPSc. Additionally, a PCR product for the Sry gene, a product specific to males, was ...Continue Reading

References

May 9, 1991·Nature·P KoopmanR Lovell-Badge
Jun 11, 1993·Nucleic Acids Research·E J Payen, C Y Cotinot
Jan 1, 1993·Microbiology and Immunology·T OnoderaM Shinagawa
Sep 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·R Griffiths, B Tiwari
Aug 9, 1997·The Veterinary Record·N HunterG Smith
Sep 2, 2000·Journal of Clinical Microbiology·K I O'RourkeD P Knowles
Mar 29, 2001·The Journal of Biological Chemistry·W TuoK I O'Rourke
Apr 18, 2002·Proceedings of the National Academy of Sciences of the United States of America·Wenbin TuoDonald P Knowles
Oct 5, 2002·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Olivier AndréolettiFrançois Schelcher
Aug 11, 2004·The Journal of General Virology·Anne BuschmannMartin H Groschup
Oct 16, 2004·The Journal of General Virology·J-Y MadecT Baron
Dec 14, 2004·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Patrick CaplaziTimothy V Baszler
Sep 22, 2005·American Journal of Veterinary Research·Jessica M EvoniukDale A Redmer

❮ Previous
Next ❯

Citations

Aug 8, 2008·Journal of Virology·James B StantonTimothy V Baszler
Feb 3, 2011·BMC Veterinary Research·Katherine I O'RourkeDavid A Schneider
Nov 25, 2011·BMC Veterinary Research·Rohana P DassanayakeKatherine I O'Rourke
Apr 5, 2012·BMC Veterinary Research·Katherine I O'RourkeThomas C Truscott
May 16, 2007·Journal of the American Veterinary Medical Association·Marie S Bulgin, Sharon Sorensen Melson
Jun 16, 2009·Biochemical and Biophysical Research Communications·James B StantonTimothy V Baszler
Nov 10, 2015·Preventive Veterinary Medicine·Giovanni MartemucciAngela Gabriella D'Alessandro
Apr 19, 2015·The Journal of General Virology·David A SchneiderKatherine I O'Rourke
May 21, 2015·ILAR Journal·Justin J Greenlee, M Heather West Greenlee
Jun 21, 2012·Biochemical and Biophysical Research Communications·Sally A Madsen-BouterseDavid A Schneider
Jan 17, 2015·Virus Research·Juan F Muñoz-GutiérrezJames B Stanton
May 2, 2009·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·David A SchneiderKatherine I O'Rourke
Sep 21, 2018·PloS One·Sally A Madsen-BouterseDavid A Schneider
Sep 12, 2018·Veterinary Pathology·Justin J Greenlee
Feb 28, 2007·The Journal of General Virology·C LacrouxO Andréoletti
Apr 6, 2019·Veterinary and Animal Science·Marta G SilvaDonald P Knowles
Jun 21, 2021·Genetics, Selection, Evolution : GSE·Sally A Madsen-BouterseWilfred Goldmann

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.