Prussian Blue Nanoparticles as a Catalytic Label in a Sandwich Nanozyme-Linked Immunosorbent Assay

Analytical Chemistry
Zdeněk FarkaPetr Skládal

Abstract

Enzyme immunoassays are widely used for detection of analytes within various samples. However, enzymes as labels suffer several disadvantages such as high production cost and limited stability. Catalytic nanoparticles (nanozymes) can be used as an alternative label in immunoassays overcoming the inherent disadvantages of enzymes. Prussian blue nanoparticles (PBNPs) are nanozymes composed of the Fe4[Fe(CN)6]3-based coordination polymer. They reveal peroxidase-like activity and are capable of catalyzing the oxidation of colorless 3,3',5,5'-tetramethylbenzidine in the presence of H2O2 to form intensely blue product. Here, we introduce the method for conjugation of PBNPs with antibodies and their application in nanozyme-linked immunosorbent assay (NLISA). Sandwich NLISA for detection of human serum albumin in urine was developed with limit of detection (LOD) of 1.2 ng·mL-1 and working range up to 1 μg·mL-1. Furthermore, the microbial contamination of Salmonella Typhimurium in powdered milk was detected with LOD of 6 × 103 colony-forming units (cfu)·mL-1 and working range up to 106 cfu·mL-1. In both cases, a critical comparison with the same immunoassay but using native peroxidase as label was realized. The achieved results confirme...Continue Reading

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Jul 29, 2017·Chemical Reviews·Zdeněk FarkaPetr Skládal

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Citations

Mar 29, 2019·Journal of Biomaterials Science. Polymer Edition·Meltem Koca EsentürkAdil Denizli
May 18, 2019·Analytical and Bioanalytical Chemistry·Zahra DehghaniMorteza Hosseini
Sep 12, 2019·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Wenshi LiXiliang Luo
Apr 1, 2020·Chembiochem : a European Journal of Chemical Biology·Yingxu ShangBaoquan Ding
May 1, 2020·Chembiochem : a European Journal of Chemical Biology·Huiyuan SunRong Yang
Jun 30, 2019·Analytical and Bioanalytical Chemistry·Ekaterina MakhnevaLenka Zajíčková
Jan 15, 2021·Comprehensive Reviews in Food Science and Food Safety·Yafang ShenYanbin Li
Sep 1, 2019·Comprehensive Reviews in Food Science and Food Safety·Lunjie HuangQingyi Wei
Jul 14, 2020·Journal of Hazardous Materials·Binesh UnnikrishnanChih-Ching Huang
Apr 30, 2021·ACS Applied Materials & Interfaces·Jie LiMin Zhang
Jul 3, 2021·International Journal of Molecular Sciences·Joan Estelrich, M Antònia Busquets
Jul 25, 2021·Biosensors & Bioelectronics·Amir M AshrafiVojtech Adam
Sep 8, 2020·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Yan LengJiayu Wan

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