PsaC subunit of photosystem I is oriented with iron-sulfur cluster F(B) as the immediate electron donor to ferredoxin and flavodoxin

Biophysical Journal
Ilya R VassilievJ H Golbeck

Abstract

The PsaC subunit of photosystem I (PS I) binds two [4Fe-4S] clusters, F(A) and F(B), functioning as electron carriers between F(X) and soluble ferredoxin. To resolve the issue whether F(A) or F(B) is proximal to F(X), we used single-turnover flashes to promote step-by-step electron transfer between electron carriers in control (both F(A) and F(B) present) and HgCl2-treated (F(B)-less) PS I complexes from Synechococcus sp. PCC 6301 and analyzed the kinetics of P700+ reduction by monitoring the absorbance changes at 832 nm in the presence of a fast electron donor (phenazine methosulfate (PMS)). In control PS I complexes exogenously added ferredoxin, or flavodoxin could be photoreduced on each flash, thus allowing P700+ to be reduced from PMS. In F(B)-less complexes, both in the presence and in the absence of ferredoxin or flavodoxin, P700+ was reduced from PMS only on the first flash and was reduced from F(X)- on the following flashes, indicating lack of electron transfer to ferredoxin or flavodoxin. In the F(B)-less complexes, a normal level of P700 photooxidation was detected accompanied by a high yield of charge recombination between P700+ and F(A)- in the presence of a slow donor, 2,6-dichlorophenol-indophenol. This recombina...Continue Reading

References

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Citations

Oct 29, 2013·Photosynthesis Research·Taras K AntalEsa Tyystjärvi
Oct 15, 2014·FEBS Letters·Marina A KozulevaBoris N Ivanov
Dec 7, 2013·Pest Management Science·Timothy R Hawkes
Nov 1, 2001·Biochimica Et Biophysica Acta·P Sétif
May 17, 2018·Photosynthesis Research·Anastasia PetrovaMarina Kozuleva
Dec 18, 2002·Annals of the New York Academy of Sciences·Nicolas RouhierJean-Pierre Jacquot
Mar 6, 1999·The Journal of Biological Chemistry·O KlukasW Saenger
Dec 18, 2002·Annals of the New York Academy of Sciences·Jean-Pierre JacquotEric Gelhaye
Nov 1, 2001·Biochimica Et Biophysica Acta·H V SchellerJ Knoetzel
Nov 1, 2001·Biochimica Et Biophysica Acta·I R VassilievJ H Golbeck
Apr 1, 2011·The Journal of Physical Chemistry. a·Peter N CiesielskiG Kane Jennings
Dec 30, 2014·Langmuir : the ACS Journal of Surfaces and Colloids·Vivek B ShahPratim Biswas
Oct 6, 2010·Biochimica Et Biophysica Acta·Andreas Busch, Michael Hippler

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