Abstract
Identification of neurons, and their phenotype, that are activated in response to specific stimuli is a critical step in understanding how neural networks integrate inputs to produce specific outputs. Here, we developed novel mouse monoclonal antibodies of different IgG isotypes that are specific to tyrosine hydroxylase (TH), and to tyrosine hydroxylase activated at its serine 40 position (pSer40TH), in order to assess changes in the activity of phenotypically identified cardiovascular neurons using fluorescence immunohistochemistry. We find that the proportion of C1 pSer40TH-positive neurons in the central and medial region of the rat rostral ventrolateral medulla (RVLM) increases dramatically following hydralazine treatment, whereas phenylephrine treatment does not significantly change the pSer40TH/TH ratio in these regions compared to control. This finding suggests that there is a mediolateral topology associated with the activation of C1 neurons following baroreceptor loading or unloading. Overall, we conclude first, that our newly characterized monoclonal antibodies are specific, and selective, against TH and pSer40TH. Secondly, that they can be used to label TH and pSer40TH immunoreactive neurons simultaneously, and third...Continue Reading
References
Mar 2, 1990·Neuroscience Letters·R M McAllen, R A Dampney
Sep 16, 1985·Neuroscience Letters·P PilowskyJ Chalmers
Oct 1, 1973·Acta Physiologica Scandinavica·T HökfeltO Johansson
Sep 10, 1984·The Journal of Comparative Neurology·C A RossD J Reis
Jan 1, 1995·Clinical and Experimental Hypertension : CHE·J B MinsonJ P Chalmers
Mar 1, 1996·Hypertension·J MinsonJ Chalmers
Apr 1, 1996·Clinical and Experimental Hypertension : CHE·J B MinsonJ P Chalmers
Dec 31, 1997·The Journal of Comparative Neurology·A M Schreihofer, P G Guyenet
Mar 28, 1998·Neuropharmacology·M J Rocha, H Herbert
Jul 8, 1998·Neuroscience Letters·J Horiuchi, R A Dampney
Mar 10, 2001·The Journal of Comparative Neurology·J K PhillipsJ Lipski
Sep 7, 2002·Hypertension·Valin RejaPaul M Pilowsky
Apr 30, 2003·The Journal of Comparative Neurology·Matthew WestonPatrice G Guyenet
May 25, 2004·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Anthony J M Verberne, Daniela M Sartor
Dec 1, 2004·Journal of Neurochemistry·Peter R DunkleyPhillip W Dickson
May 12, 2005·Neuroscience·D A SpringellA K Goodchild
Nov 3, 2005·The European Journal of Neuroscience·Deborah A SpringellAnn K Goodchild
Aug 10, 2006·Clinical and Experimental Pharmacology & Physiology·Valin RejaPaul M Pilowsky
May 19, 2007·Brain, Behavior and Evolution·K W S AshwellC R R Watson
Jul 8, 2008·Journal of Neuroendocrinology·K J Kovács
Apr 2, 2009·Antioxidants & Redox Signaling·Gayatri RaghuramanGanesh K Kumar
Aug 5, 2009·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Paul M PilowskySimon McMullan
Jul 29, 2011·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Peter G R BurkeAnn K Goodchild
Jul 30, 2011·The Journal of Comparative Neurology·Darko SpirovskiPaul M Pilowsky
Dec 5, 2012·PloS One·Hanafi A DamanhuriAnn K Goodchild
Oct 9, 2013·Brain Structure & Function·Lindsay M ParkerAnn K Goodchild
Citations
May 8, 2016·Neuroscience·Komal KapoorPaul M Pilowsky
Jun 5, 2016·Autonomic Neuroscience : Basic & Clinical·Komal KapoorPaul M Pilowsky
Jul 8, 2016·The Journal of Pharmacology and Experimental Therapeutics·Seung Jae KimMelissa M J Farnham
Mar 11, 2018·Experimental Physiology·Mamoru TanidaToshishige Shibamoto
Aug 24, 2018·The Journal of Comparative Neurology·M SenthilkumaranI J Llewellyn-Smith
Sep 10, 2019·Frontiers in Neuroscience·Melissa M J FarnhamPaul M Pilowsky
Oct 20, 2020·Pflügers Archiv : European journal of physiology·Ahmed A RahmanKulmira Nurgali
Mar 23, 2021·Frontiers in Physiology·Polina E NedoboyMelissa M J Farnham
Jul 14, 2021·Scientific Reports·Sheran LiJacqueline K Phillips
Aug 28, 2021·Nutrients·Polina E NedoboyMelissa M-J Farnham