pSer40 tyrosine hydroxylase immunohistochemistry identifies the anatomical location of C1 neurons in rat RVLM that are activated by hypotension

Neuroscience
P E NedoboyPaul M Pilowsky

Abstract

Identification of neurons, and their phenotype, that are activated in response to specific stimuli is a critical step in understanding how neural networks integrate inputs to produce specific outputs. Here, we developed novel mouse monoclonal antibodies of different IgG isotypes that are specific to tyrosine hydroxylase (TH), and to tyrosine hydroxylase activated at its serine 40 position (pSer40TH), in order to assess changes in the activity of phenotypically identified cardiovascular neurons using fluorescence immunohistochemistry. We find that the proportion of C1 pSer40TH-positive neurons in the central and medial region of the rat rostral ventrolateral medulla (RVLM) increases dramatically following hydralazine treatment, whereas phenylephrine treatment does not significantly change the pSer40TH/TH ratio in these regions compared to control. This finding suggests that there is a mediolateral topology associated with the activation of C1 neurons following baroreceptor loading or unloading. Overall, we conclude first, that our newly characterized monoclonal antibodies are specific, and selective, against TH and pSer40TH. Secondly, that they can be used to label TH and pSer40TH immunoreactive neurons simultaneously, and third...Continue Reading

References

Oct 1, 1973·Acta Physiologica Scandinavica·T HökfeltO Johansson
Jan 1, 1995·Clinical and Experimental Hypertension : CHE·J B MinsonJ P Chalmers
Apr 1, 1996·Clinical and Experimental Hypertension : CHE·J B MinsonJ P Chalmers
Dec 31, 1997·The Journal of Comparative Neurology·A M Schreihofer, P G Guyenet
Mar 10, 2001·The Journal of Comparative Neurology·J K PhillipsJ Lipski
Sep 7, 2002·Hypertension·Valin RejaPaul M Pilowsky
Apr 30, 2003·The Journal of Comparative Neurology·Matthew WestonPatrice G Guyenet
May 25, 2004·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Anthony J M Verberne, Daniela M Sartor
Dec 1, 2004·Journal of Neurochemistry·Peter R DunkleyPhillip W Dickson
Nov 3, 2005·The European Journal of Neuroscience·Deborah A SpringellAnn K Goodchild
Aug 10, 2006·Clinical and Experimental Pharmacology & Physiology·Valin RejaPaul M Pilowsky
May 19, 2007·Brain, Behavior and Evolution·K W S AshwellC R R Watson
Jul 8, 2008·Journal of Neuroendocrinology·K J Kovács
Aug 5, 2009·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Paul M PilowskySimon McMullan
Jul 29, 2011·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Peter G R BurkeAnn K Goodchild

❮ Previous
Next ❯

Citations

Jun 5, 2016·Autonomic Neuroscience : Basic & Clinical·Komal KapoorPaul M Pilowsky
Jul 8, 2016·The Journal of Pharmacology and Experimental Therapeutics·Seung Jae KimMelissa M J Farnham
Aug 24, 2018·The Journal of Comparative Neurology·M SenthilkumaranI J Llewellyn-Smith
Sep 10, 2019·Frontiers in Neuroscience·Melissa M J FarnhamPaul M Pilowsky
Mar 23, 2021·Frontiers in Physiology·Polina E NedoboyMelissa M J Farnham

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antihypertensive Agents: Mechanisms of Action

Antihypertensive drugs are used to treat hypertension (high blood pressure) which aims to prevent the complications of high blood pressure, such as stroke and myocardial infarction. Discover the latest research on antihypertensive drugs and their mechanism of action here.