PMID: 7538151Feb 1, 1995Paper

"Pseudoclusters" and typing by random amplification of polymorphic DNA of Aspergillus fumigatus

Journal of Clinical Pathology
K W LoudonJ P Burnie

Abstract

This short report serves as a warning to the unwary of possible "pseudoclusters" of infection with Aspergillus fumigatus as shown by the typing system, random amplification of polymorphic DNA (RAPD). This was demonstrated by typing 10 epidemiologically distinct isolates of A fumigatus using two different preparations of Taq DNA polymerase. One of the enzymes did not discriminate between the isolates, giving the false impression that a cluster of infection had occurred. Enzyme source is thus a key variable when using RAPD to distinguish between isolates of A fumigatus.

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Jan 1, 1994·Journal of Medical and Veterinary Mycology : Bi-monthly Publication of the International Society for Human and Animal Mycology·K W LoudonJ A Liu Yin
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Citations

Jun 12, 2003·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·János Varga, Beáta Tóth
May 2, 2000·Applied and Environmental Microbiology·S M Cusick, D J O'Sullivan
Feb 7, 2001·Clinical Microbiology Reviews·D R Soll
May 28, 2019·FEMS Microbiology Letters·Rooholla GhanbariEbrahim Molaee Aghaee
Apr 9, 1999·Clinical Microbiology Reviews·J P Latgé
Aug 1, 1997·Infection and Immunity·J P DebeaupuisJ P Latgé
Dec 1, 1995·Journal of Clinical Microbiology·K W Loudon, J P Burnie

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