PMID: 8599759Apr 1, 1996Paper

Pseudocontact shifts used in the restraint of the solution structures of electron transfer complexes

Nature Structural Biology
R D GuilesL Waskell

Abstract

The geometry of the ferricytochrome b5-ferricytochrome c complex has been analysed using long-range interprotein paramagnetic dipolar shifts. Heteronuclear filtered NMR spectra of samples containing 15N-labelled cytochrome b5 in complex with unlabelled cytochrome c allowed unambiguous assessment of pseudocontact shifts relative to diamagnetic reference states. Because pseudocontact shifts can be observed for protons as much as 20 A from the paramagnetic centre, this approach allows study of electron transfer proteins in fast exchange. Our findings provide the first physical evidence confirming hypotheses presented in previous theoretical studies. This absence of certain predicted shifts that are expected based on the best fit to a static model of the complex suggests that cytochrome b5 is more dynamic in solution than in the crystal, in agreement with molecular dynamics simulations.

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Citations

Jan 1, 1996·Progress in Biophysics and Molecular Biology·I BertiniA Rosato
Sep 19, 2002·Protein Science : a Publication of the Protein Society·Brian J GoodfellowJohn L Markley
Jul 1, 1998·Annual Review of Biophysics and Biomolecular Structure·K H Gardner, L E Kay
Sep 24, 2004·Journal of Molecular Recognition : JMR·Miguel Prudêncio, Marcellus Ubbink
Feb 4, 2005·Protein Science : a Publication of the Protein Society·Alexander N VolkovMarcellus Ubbink
May 4, 2002·Annual Review of Biophysics and Biomolecular Structure·M UbbinkM Huber
Aug 16, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Rui M AlmeidaSofia R Pauleta
Apr 2, 2005·Protein Expression and Purification·Ashima MitraSiddhartha P Sarma
Aug 12, 2005·Chembiochem : a European Journal of Chemical Biology·Ivano BertiniRoberta Pierattelli
Nov 28, 2007·Biopolymers·Juliette T J LecomteMatthew P Pond
Jul 29, 2003·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Lucia BanciAntonio Rosato
Jun 14, 2005·Progress in Biophysics and Molecular Biology·Pedro M MatiasMaria Arménia Carrondo
Jun 9, 2005·Chemical Reviews·Gérard Simonneaux, Arnaud Bondon
May 27, 2003·Biochimica Et Biophysica Acta·Lars J C Jeuken
Sep 8, 2010·Archives of Biochemistry and Biophysics·Irina F Sevrioukova, Thomas L Poulos

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