PMID: 8955900Jan 1, 1996Paper

Pseudoknot and translational control in the expression of the S15 ribosomal protein

Biochimie
L BénardC Portier

Abstract

Translational autocontrol of the expression of the ribosomal protein S15 proceeds through the transitory formation of a pseudoknot. A synopsis of the known data is used to propose a molecular model of the mechanism involved and for the role of the pseudoknot. This latter structure is able to recruit 30S ribosomal subunits to initiate translation, but also to bind S15 and to stop translation by trapping the ribosome on its loading site. Information on the S15 protein recognition of the messenger RNA site was deduced from mutational analyses and chemical probing. A comparison of this messenger site with the S15 ribosomal binding site was conducted by analysing hydroxyl radical footprintings of these two sites. The existence of two subsites in 16S RNA suggests that the ribosomal protein S15 might present either two different binding sites or at least one common subsite. Clues for the presence of a common site between the messenger and 16S RNA are given which cannot rule out that recognition specificity is linked to a few other determinants. Whether these determinants are different or not remains an open question.

References

Jul 30, 1990·Journal of Molecular Biology·J D PuglisiI Tinoco
Jul 30, 1990·Journal of Molecular Biology·J R WyattI Tinoco
Aug 27, 1990·Biochimica Et Biophysica Acta·C PortierC Ehresmann
Jan 20, 1990·Journal of Molecular Biology·C PortierM Grunberg-Manago
Dec 23, 1987·Nucleic Acids Research·R C Conrad, G R Craven
Sep 29, 1995·The Journal of Biological Chemistry·A M Baker, D E Draper
Feb 5, 1993·Journal of Molecular Biology·G SpeddingD E Draper
May 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·C PhilippeC Ehresmann

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Citations

May 16, 1998·Proceedings of the National Academy of Sciences of the United States of America·Y ChoquetF A Wollman
Dec 9, 2009·Nucleic Acids Research·Jonathan Livny, Matthew K Waldor
Mar 10, 2005·Microbiology and Molecular Biology Reviews : MMBR·Brian Søgaard LaursenHans Uffe Sperling-Petersen
Jun 6, 2003·Molecular Microbiology·Paula Jean Schlax, David J Worhunsky

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