Pseudomonas aeruginosa elastase does not inactivate alpha 1-proteinase inhibitor in the presence of leukocyte elastase.

Infection and Immunity
M Padrines, J G Bieth

Abstract

Pseudomonas aeruginosa elastase rapidly inactivates alpha 1-proteinase inhibitor by splitting its Pro-357-Met-358 peptide bond. The present study was aimed at testing whether this reaction takes place in the presence of leukocyte elastase. To this end was added alpha 1-proteinase inhibitor to a mixture of the two elastases, and we performed the following assays: (i) measurement of the residual leukocyte elastase activity, (ii) sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and (iii) immunoassay of the leukocyte elastase-alpha 1-proteinase inhibitor complex. These experiments were done with various concentrations of the three proteins. All experiments gave the same result: leukocyte elastase was fully inhibited by alpha 1-proteinase inhibitor in the presence of P. aeruginosa elastase even when the bacterial enzyme was 10-fold more concentrated than the neutrophil enzyme. We also measured the initial rate of the P. aeruginosa elastase-catalyzed inactivation of alpha 1-proteinase inhibitor as a function of the inhibitor concentration. The kcat/Km value derived from this experiment was 9 x 10(4) M-1 s-1, a value much lower than the rate constant for the leukocyte elastase-inhibitor association (kass, 1.7 x 10(7) M-1 s-1...Continue Reading

References

Mar 1, 1989·The American Review of Respiratory Disease·M PadrinesJ G Bieth
Mar 16, 1989·Biochimica Et Biophysica Acta·C Boudier, J G Bieth
Apr 1, 1987·The American Review of Respiratory Disease·A HamdaouiJ G Bieth
Apr 1, 1987·Biological Chemistry Hoppe-Seyler·N J BraunH P Schnebli
Dec 1, 1965·Experimental and Molecular Pathology·J D Mull, W S Callahan
Jan 1, 1983·Annual Review of Biochemistry·J Travis, G S Salvesen
Nov 1, 1983·Reviews of Infectious Diseases·B Wretlind, O R Pavlovskis
Jan 1, 1965·Annual Review of Biochemistry·M L BENDER, J KEZDY
Nov 30, 1967·Biochemical and Biophysical Research Communications·T Chase, E Shaw

❮ Previous
Next ❯

Citations

Feb 1, 1991·American Journal of Respiratory Cell and Molecular Biology·M Padrines, J G Bieth
Feb 1, 1992·Journal of Paediatrics and Child Health·K Grimwood
Jan 1, 1991·Annals of the New York Academy of Sciences·S I RennardA B Thompson
Dec 1, 1994·American Journal of Respiratory and Critical Care Medicine·S Suter
Mar 7, 2021·Drug Discovery Today·Martin J Everett, David T Davies

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.