Pseudomonas aeruginosa exoenzyme S induces proliferation of human T lymphocytes.

Infection and Immunity
C H ModyD E Woods

Abstract

Pseudomonas aeruginosa is a gram-negative bacterium that is responsible for devastating acute and chronic infections, which include bronchiectasis in cystic fibrosis, nosocomial pneumonia, and infection of burn wounds. Previous studies have demonstrated that these patients have impaired host responses, including cell-mediated immune responses, which are important in anti-Pseudomonas host defense. The P. aeruginosa exoproduct, exoenzyme S, has a number of characteristics which suggest that it might be important in cell-mediated immunity. To determine whether exoenzyme S activates lymphocytes to proliferate, peripheral blood mononuclear cells (PBMC) from normal volunteers were stimulated with purified exoenzyme S, and the lymphocyte response was assessed by measuring [3H]thymidine uptake and by counting the number of cells after various times in culture. Ninety-five percent of healthy adult donors had a lymphocyte response to exoenzyme S. The optimal lymphocyte response occurred on day 7, with 4 x 10(5) PBMC per microtiter well when cells were stimulated with 10 micrograms exoenzyme S per ml. [3H]thymidine uptake correlated with an increase in the number of mononuclear cells, indicating that proliferation occurred. In unseparated...Continue Reading

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Citations

Jul 22, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Slava EpelmanChristopher H Mody
Jun 10, 2008·American Journal of Respiratory Cell and Molecular Biology·Slava EpelmanChristopher H Mody
Jan 15, 1996·FEMS Microbiology Letters·J Goranson, D W Frank
May 26, 2016·Current Infectious Disease Reports·Aric L Gregson
Jan 1, 1997·Critical Reviews in Microbiology·C Hardalo, S C Edberg
Aug 17, 2020·Annals of the American Thoracic Society·Jaskaran SethiNirmal S Sharma

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