Nov 28, 2017

Pseudomonas aeruginosa exoproducts determine antibiotic efficacy against Staphylococcus aureus

PLoS Biology
Lauren RadlinskiBrian P Conlon

Abstract

Chronic coinfections of Staphylococcus aureus and Pseudomonas aeruginosa frequently fail to respond to antibiotic treatment, leading to significant patient morbidity and mortality. Currently, the impact of interspecies interaction on S. aureus antibiotic susceptibility remains poorly understood. In this study, we utilize a panel of P. aeruginosa burn wound and cystic fibrosis (CF) lung isolates to demonstrate that P. aeruginosa alters S. aureus susceptibility to bactericidal antibiotics in a variable, strain-dependent manner and further identify 3 independent interactions responsible for antagonizing or potentiating antibiotic activity against S. aureus. We find that P. aeruginosa LasA endopeptidase potentiates lysis of S. aureus by vancomycin, rhamnolipids facilitate proton-motive force-independent tobramycin uptake, and 2-heptyl-4-hydroxyquinoline N-oxide (HQNO) induces multidrug tolerance in S. aureus through respiratory inhibition and reduction of cellular ATP. We find that the production of each of these factors varies between clinical isolates and corresponds to the capacity of each isolate to alter S. aureus antibiotic susceptibility. Furthermore, we demonstrate that vancomycin treatment of a S. aureus mouse burn infecti...Continue Reading

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Citations

Mentioned in this Paper

Pathologic Cytolysis
Vancomycin
Microorganism
Pseudomonas Infections
Study
AB-Vancomycin
Bactericide, NOS
Cratoxylum formosum
Cellular Response to ATP
Morbidity Aspects

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