Pseudomonas aeruginosa ExoS and ExoT

Reviews of Physiology, Biochemistry and Pharmacology
J T Barbieri, Jianjun Sun

Abstract

ExoS and ExoT are bi-functional type-III cytotoxins of Pseudomonas aeruginosa that share 76% primary amino acid homology and contain N-terminal RhoGAP domains and C-terminal ADP-ribosylation domains. The Rho GAP activities of ExoS and ExoT appear to be biochemically and biologically identical, targeting Rho, Rac, and Cdc42. Expression of the RhoGAP domain in mammalian cells results in the disruption of the actin cytoskeleton and interference of phagocytosis. Expression of the ADP-ribosyltransferase domain of ExoS elicits a cytotoxic phenotype in cultured cells, while expression of ExoT appears to interfere with host cell phagocytic activity. Recent studies showed that ExoS and ExoT ADP-ribosylate different substrates. While ExoS has poly-substrate specificity and can ADP-ribosylate numerous host proteins, ExoT ADP-ribosylates a more restricted subset of host proteins including the Crk proteins. Protein modeling predicts that electrostatic interactions contribute to the substrate specificity of the ADP-ribosyltransferase domains of ExoS and ExoT.

Citations

Jul 24, 2010·Amino Acids·Sabrina LaingFriedrich Haag
Mar 11, 2005·Nature Reviews. Microbiology·Timothy L Cover, Steven R Blanke
Aug 15, 2009·Nature Reviews. Microbiology·Alan R Hauser
Dec 12, 2007·The Journal of Experimental Medicine·Fayyaz S SutterwalaRichard A Flavell
Mar 28, 2009·Critical Care Medicine·Jeffrey L VeesenmeyerJordi Rello
May 1, 2013·Infection and Immunity·Sushmita MustafiM Alejandro Barbieri
Sep 13, 2006·Infection and Immunity·Jinghua JiaShouguang Jin
Jan 27, 2010·Infection and Immunity·Maureen H Diaz, Alan R Hauser
May 11, 2010·Journal of Bacteriology·Christopher A VakulskasTimothy L Yahr
Jul 10, 2013·Journal of Bacteriology·Alicia E Ballok, George A O'Toole
Sep 1, 2009·Journal of Bacteriology·Christopher A VakulskasTimothy L Yahr
Jan 24, 2007·Journal of Bacteriology·Yael Litvak, Zvi Selinger
Nov 28, 2007·Journal of Bacteriology·Michelle CiszArne Rietsch
Jan 1, 2008·Journal of Bacteriology·Joanna B GoldbergPierre Cornelis
Feb 3, 2006·Journal of Bacteriology·S FurukawaG A O'Toole
Jan 20, 2007·The Journal of Clinical Investigation·Priya BalachandranJoanne Engel
Apr 27, 2010·BMC Microbiology·Daniel SperandioAnnabelle Merieau
Oct 11, 2012·PloS One·F Jerry ReenFergal O'Gara
Jan 12, 2013·Current Protein & Peptide Science·Marlies GalleRudi Beyaert
Apr 26, 2007·Proceedings of the National Academy of Sciences of the United States of America·John F RawlsJeffrey I Gordon
Oct 13, 2006·Proceedings of the National Academy of Sciences of the United States of America·Sasha H Shafikhani, Joanne Engel
Jun 30, 2005·Proceedings of the National Academy of Sciences of the United States of America·Mark L UrbanowskiTimothy L Yahr
Aug 20, 2014·Proceedings of the National Academy of Sciences of the United States of America·Thorsten EierhoffWinfried Römer
Jan 26, 2016·Journal of Medicinal Chemistry·Stefanie WagnerAlexander Titz
Jul 16, 2005·Annals of Medicine·Luís J Mota, Guy R Cornelis
Sep 26, 2006·Molecular Microbiology·Timothy L Yahr, Matthew C Wolfgang

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