Pseudomonas aeruginosa-induced infection and degradation of human wound fluid and skin proteins ex vivo are eradicated by a synthetic cationic polymer

The Journal of Antimicrobial Chemotherapy
M WerthénArtur Schmidtchen

Abstract

Antimicrobial peptides are important effectors of innate immunity. Bacteria display multiple defence mechanisms against these peptides. For example, Pseudomonas aeruginosa releases potent proteinases that inactivate the human cathelicidin LL-37. Hence, in conditions characterized by persistent bacterial colonization, such as in P. aeruginosa-infected skin wounds, there is a need for efficient means of reducing bacterial load. Here, the effect of the cationic molecule polyhexamethylenebiguanide (PHMB) was evaluated. Infection models in human wound fluid and human skin were established. Radial diffusion methods, bacterial growth and bactericidal assays were used for determination of effects of PHMB on bacteria in the presence of plasma, wound fluid or human skin. At the protein and tissue levels, SDS-PAGE, light microscopy and scanning electron microscopy were used to study the effects of P. aeruginosa infection before and after addition of PHMB. PHMB killed common ulcer-derived bacteria in the presence of human wound fluid. Furthermore, elastase-expressing P. aeruginosa completely degraded wound fluid proteins as well as human skin during infection ex vivo. The infection, and consequent protein degradation, was reversed by PHMB....Continue Reading

References

Feb 1, 1992·Ophthalmology·D F LarkinJ K Dart
Mar 21, 1991·Journal of Immunological Methods·R I LehrerP Eisenhauer
Aug 1, 1984·The Journal of Applied Bacteriology·P BroxtonP Gilbert
Feb 27, 1999·Current Opinion in Immunology·R I Lehrer, T Ganz
Jul 15, 1999·The International Journal of Biochemistry & Cell Biology·J M Schröder, J Harder
Mar 17, 2000·Immunological Reviews·H G Boman
Jul 27, 2001·Nature·S Fernandez-LopezM R Ghadiri
Jan 25, 2002·Nature·Michael Zasloff
Feb 28, 2002·Acta Dermato-venereologica·A SchmidtchenC Hansson
Mar 19, 2002·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·C E DaviesD W Thomas
Oct 9, 2002·Molecular Microbiology·Artur SchmidtchenLars Björck
Oct 11, 2002·The New England Journal of Medicine·Peck Y OngDonald Y M Leung
Aug 22, 2003·Journal of Internal Medicine·H G Boman
Mar 1, 1995·Trends in Cell Biology·M E Selsted, A J Ouellette

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Citations

Apr 14, 2010·Antimicrobial Agents and Chemotherapy·Yong Pyo ShinIn Hee Lee
Feb 8, 2011·PloS One·Martin MalmstenArtur Schmidtchen
Aug 28, 2014·Critical Reviews in Microbiology·Steven L PercivalBenjamin A Lipsky
May 17, 2011·Journal of Controlled Release : Official Journal of the Controlled Release Society·Nicolas BertrandJeanne Leblond
Sep 28, 2015·Journal of Controlled Release : Official Journal of the Controlled Release Society·Jing LiDavid Oupický
May 24, 2006·Biochimica Et Biophysica Acta·Ulrich H N DürrAyyalusamy Ramamoorthy
Jul 15, 2015·Advances in Wound Care·Simon Finnegan, Steven L Percival
Nov 26, 2009·Natural Product Reports·Matthew F Burton, Patrick G Steel
Mar 15, 2014·The Open Microbiology Journal·Hosan KimMina Izadjoo
Sep 15, 2009·British Journal of Community Nursing·Karen Ousey, Caroline McIntosh
Jul 19, 2012·Health & Social Care in the Community·Geoffrey K MitchellAlison Love
Aug 10, 2012·British Journal of Nursing : BJN·Martyn Butcher
Dec 21, 2017·Skin Pharmacology and Physiology·Axel KramerOjan Assadian
Mar 15, 2020·Metabolomics : Official Journal of the Metabolomic Society·Moamen M ElmassryAbdul N Hamood
Oct 7, 2015·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Guangxue FengBin Liu
Jun 3, 2021·International Journal of Molecular Sciences·Madelene Å AnderssonManoj Puthia
Nov 12, 2021·Parasite : Journal De La Société Française De Parasitologie·Jian-Ming HuangFu-Chin Huang

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