Pseudomonas aeruginosa Inhibits the Growth of Scedosporium and Lomentospora In Vitro

Mycopathologia
Sharon C-A ChenTania C Sorrell

Abstract

In vitro bacterial-fungal interaction studies in cystic fibrosis (CF) have mainly focused on interactions between bacteria and Candida. Here we investigated the effect of Pseudomonas aeruginosa on the growth of Scedosporium/Lomentospora spp. Standard suspensions of P. aeruginosa (16 non-mucoid and nine mucoid isolates) were dropped onto paper disks, placed on lawns of Lomentospora prolificans (formerly Scedosporium prolificans) strain WM 14.140 or Scedosporium aurantiacum strain WM 11.78 on solid agar. The median inhibitory activity (mIz) was calculated for each fungal-bacterial combination. As a group, mIz values for non-mucoid phenotype P. aeruginosa strains were significantly lower than those for mucoid strains (P < 0.001); 14/16 (87.5%) non-mucoid strains had mIz <1.0 against both fungi versus just 3/9 mucoid strains (33.4%) (P = 0.01). One non-mucoid (PA14) and one mucoid (CIDMLS-PA-28) P. aeruginosa strain effecting inhibition were selected for further studies. Inhibition of both L. prolificans and S. aurantiacum by these strains was confirmed using the XTT (2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino)carbonyl]-2H-tetrazolium hydroxide) reduction assay. Following incubation with XTT, inhibition of fungal grow...Continue Reading

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Citations

Jul 12, 2018·Pediatric Pulmonology·Michael C Tracy, Richard B Moss
Oct 13, 2018·Annual Review of Medicine·Michael M ReyDenis Hadjiliadis
Dec 24, 2019·Expert Review of Respiratory Medicine·Jean-Philippe BoucharaGilles Nevez
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May 24, 2019·Pathogens·Wibke KrügerMaria Joanna Niemiec
Aug 28, 2020·Frontiers in Microbiology·Hai-Yue LiuJin Su
Jan 20, 2018·Mycopathologia·Jean-Philippe BoucharaVishnu Chaturvedi
Dec 1, 2020·Frontiers in Cellular and Infection Microbiology·Evely Bertulino de OliveiraEliana Barreto-Bergter
Jan 12, 2021·Expert Opinion on Drug Delivery·Douweh Leyla Gbian, Abdelwahab Omri
Jan 15, 2021·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Andressa de Jesus MarquesLivia Cristina Liporagi-Lopes
Oct 19, 2019·Microorganisms·Filomena NogueiraThomas Lion

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Methods Mentioned

BETA
fluorescence microscopy

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