Pseudomonas aeruginosa lipopolysaccharide induces CF-like alteration of protein secretion by human tracheal gland cells

Biochemical and Biophysical Research Communications
W KammouniM D Merten

Abstract

Human tracheal gland (HTG) serous cells are now believed to play a major role in the physiopathology of cystic fibrosis. Because of the persistent inflammation and the specific infection by Pseudomonas aeruginosa in the lung, we looked for the action of the lipopolysaccharide (LPS) of this bacteria on human tracheal gland cells in culture by studying the secretion of the secretory leukocyte proteinase inhibitor (SLPI) which is a specific serous secretory marker of these cells. Treatment with Pseudomonas aeruginosa LPS resulted in a significant dose-dependent increase in the basal production of SLPI (+ 250 +/- 25%) whilst the SLPI transcript mRNA levels remained unchanged. This LPS-induced increase in secretion was inhibited by glucocorticoides. Furthermore, LPS treatment of HTG cells induces a loss of responsiveness to carbachol and isoproterenol but not to adenosine triphosphate. These findings indicate that HTG cells treated by Pseudomonas aeruginosa LPS have the same behavior as those previously observed with CF-HTG cells. Exploration by using reverse transcriptase polymerase chain reaction amplification showed that LPS downregulated cystic fibrosis transmembrane conductance regulator (CFTR) mRNA expression in HTG cells indi...Continue Reading

Citations

Dec 19, 2002·European Journal of Clinical Investigation·A M KamalS F Smith
Apr 6, 2002·American Journal of Respiratory and Critical Care Medicine·Marianne S Muhlebach, Terry L Noah
Apr 1, 1999·American Journal of Respiratory Cell and Molecular Biology·W KammouniM D Merten
Jul 18, 2015·Mediators of Inflammation·Matthew S TwiggSinéad Weldon
Mar 31, 2016·American Journal of Respiratory and Critical Care Medicine·Jennifer A BartlettPaul B McCray
Jan 12, 2017·International Journal of Molecular Sciences·Carla M P Ribeiro, Bob A Lubamba
Aug 17, 2004·The American Journal of Gastroenterology·Andres GelrudSteven D Freedman
Dec 17, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Sinéad WeldonClifford C Taggart
Dec 9, 2016·Biological Chemistry·Donna M SmallClifford C Taggart
Jan 25, 2000·American Journal of Physiology. Lung Cellular and Molecular Physiology·S van WeteringP S Hiemstra

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