PMID: 2502533Aug 1, 1989Paper

Pseudomonas aeruginosa outer membrane lipoprotein I gene: molecular cloning, sequence, and expression in Escherichia coli

Journal of Bacteriology
M DuchêneH Domdey

Abstract

Lipoprotein I (OprI) is one of the major proteins of the outer membrane of Pseudomonas aeruginosa. Like porin protein F (OprF), it is a vaccine candidate because it antigenically cross-reacts with all serotype strains of the International Antigenic Typing Scheme. Since lipoprotein I was expressed in Escherichia coli under the control of its own promoter, we were able to isolate the gene by screening a lambda EMBL3 phage library with a mouse monoclonal antibody directed against lipoprotein I. The monocistronic OprI mRNA encodes a precursor protein of 83 amino acid residues including a signal peptide of 19 residues. The mature protein has a molecular weight of 6,950, not including bound glycerol and lipid. Although the amino acid sequences of protein I of P. aeruginosa and Braun's lipoprotein of E. coli differ considerably (only 30.1% identical amino acid residues), peptidoglycan in E. coli, are identical. Using lipoprotein I expressed in E. coli, it can now be tested whether this protein alone, without P. aeruginosa lipopolysaccharide contaminations, has a protective effect against P. aeruginosa infections.

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Citations

Jul 1, 1990·Molecular Microbiology·R E HancockN Martin
Jun 1, 1990·Journal of Bioenergetics and Biomembranes·S Hayashi, H C Wu
Nov 6, 2012·Journal of Bacteriology·Aimee K WesselMarvin Whiteley
Jan 10, 2018·Molecular Microbiology·Alexander J F Egan
Feb 6, 1998·FEMS Microbiology Reviews·E S Stanislavsky, J S Lam
Mar 1, 2014·Expert Review of Vaccines·Gregory P Priebe, Joanna B Goldberg
Oct 16, 2015·The Journal of Biological Chemistry·Samantha M DesmaraisKerwyn Casey Huang
Jan 27, 2010·The Journal of Biological Chemistry·Yu-Min LinYou-Di Liao
Apr 6, 2006·The Journal of Biological Chemistry·Etsuko SugawaraHiroshi Nikaido

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