PMID: 2498289Jun 1, 1989Paper

Pseudomonas aeruginosa outer membrane protein F: structural role and relationship to the Escherichia coli OmpA protein

Journal of Bacteriology
W A Woodruff, R E Hancock

Abstract

A Pseudomonas aeruginosa outer membrane protein F-deficient omega-insertion mutant strain H636, in contrast to its protein F-sufficient parent strain H103, was unable to grow on unsupplemented Proteose Peptone no. 2 broth (Difco Laboratories, Detroit, Mich.). Addition of high concentrations of NaCl, KCl, glucose, sucrose, or potassium succinate permitted growth of strain H636 at rates approaching those of the parent strain H103. Strain H636 cells were 33% shorter and had a 46% smaller cross-sectional area than did the parent strain growing at similar rates on the same medium. These properties of the oprF::omega mutant were analogous to those previously observed for Escherichia coli ompA mutants in an lpp (Braun lipoprotein-deficient) mutant background. Therefore, we compared P. aeruginosa protein F and the E. coli OmpA protein. In addition to many similarities previously described, sequence alignment demonstrated substantial amino acid sequence homology throughout the carboxy-terminal 168 to 180 amino acids of the two proteins. Consistent with this observation, polyclonal antiserum specific for OmpA reacted on Western blots (immunoblots) with protein F. Expression of protein F from the cloned oprF gene in an E. coli ompA lpp do...Continue Reading

References

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Citations

Feb 6, 1998·FEMS Microbiology Reviews·E S Stanislavsky, J S Lam
Sep 20, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Tim O HircheAzzaq Belaaouaj
Apr 21, 2011·Molecular Biology and Evolution·Josselin BodilisSylvie Barray
Jan 14, 2012·The FEBS Journal·Etsuko SugawaraHiroshi Nikaido
Mar 20, 2014·Molecular & Cellular Proteomics : MCP·Biswajit KhatuaChitra Mandal
Dec 6, 2016·Frontiers in Microbiology·Rahul MittalXue Z Liu
Jul 1, 1990·Molecular Microbiology·R E HancockN Martin
Apr 6, 2006·The Journal of Biological Chemistry·Etsuko SugawaraHiroshi Nikaido
Dec 30, 2010·Infection and Immunity·Laurène Fito-BoncompteSylvie Chevalier
Mar 15, 2016·Frontiers in Microbiology·Rahul MittalXue Z Liu
Jan 26, 2005·FEMS Microbiology Letters·Sebastian BeckEckhard Strauch
Aug 18, 2005·Environmental Microbiology·Maria L MarcoSteven E Lindow
Apr 1, 2009·Antimicrobial Agents and Chemotherapy·Andreas DötschSusanne Häussler
Jul 2, 2020·Antibodies·Adinarayana KunamneniRavi Durvasula
Jan 1, 1996·Protein Science : a Publication of the Protein Society·C Stathopoulos
Jan 22, 2005·Journal of Bacteriology·Keiji NaganoFuminobu Yoshimura
Mar 22, 2006·Microbiology·Josselin Bodilis, Sylvie Barray
Oct 1, 1993·Molecular Microbiology·Rebecca S Y WongRobert E W Hancock

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