Pseudomonas aeruginosa PAO1 exopolysaccharides are important for mixed species biofilm community development and stress tolerance

Frontiers in Microbiology
Saravanan PeriasamyScott A Rice

Abstract

Pseudomonas aeruginosa PAO1 produces three polysaccharides, alginate, Psl, and Pel that play distinct roles in attachment and biofilm formation for monospecies biofilms. Considerably less is known about their role in the development of mixed species biofilm communities. This study has investigated the roles of alginate, Psl, and Pel during biofilm formation of P. aeruginosa in a defined and experimentally informative mixed species biofilm community, consisting of P. aeruginosa, Pseudomonas protegens, and Klebsiella pneumoniae. Loss of the Psl polysaccharide had the biggest impact on the integration of P. aeruginosa in the mixed species biofilms, where the percent composition of the psl mutant was significantly lower (0.06%) than its wild-type (WT) parent (2.44%). In contrast, loss of the Pel polysaccharide had no impact on mixed species biofilm development. Loss of alginate or its overproduction resulted in P. aeruginosa representing 8.4 and 18.11%, respectively, of the mixed species biofilm. Dual species biofilms of P. aeruginosa and K. pneumoniae were not affected by loss of alginate, Pel, or Psl, while the mucoid P. aeruginosa strain achieved a greater biomass than its parent strain. When P. aeruginosa was grown with P. prot...Continue Reading

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Citations

May 14, 2016·Journal of Molecular Biology·Wei Hong TayKimberly A Kline
Jul 30, 2016·Microbial Biotechnology·Scott A RiceStaffan Kjelleberg
Nov 24, 2016·Environmental Microbiology·Chuan Hao TanScott A Rice
Apr 8, 2017·FEMS Microbiology Ecology·Morgan PetrovichGeorge F Wells
Oct 10, 2018·Microbiology·Viviana Teresa OrlandiRoberta Fruttero
Sep 3, 2019·Environmental Technology·Kurumi HashimotoWataru Nishijima
Apr 6, 2018·Clinical Microbiology Reviews·Maria MaganaGeorge P Tegos
Jun 2, 2020·Frontiers in Cellular and Infection Microbiology·Allison L Welp, Jennifer M Bomberger
Apr 20, 2019·Frontiers in Cellular and Infection Microbiology·Malika MoussouniAnne-Béatrice Blanc-Potard
Jul 21, 2020·Pharmaceutical Research·Aram MohammedHisham Al-Obaidi
Dec 29, 2020·Computational and Structural Biotechnology Journal·Johan Bengtsson-Palme
Mar 3, 2021·Critical Reviews in Microbiology·Faizan Ahmed SadiqHao Zhang
Mar 6, 2021·Critical Reviews in Food Science and Nutrition·Unji KimSe-Wook Oh
Mar 7, 2021·Microorganisms·Zaira Heredia-PonceJosé Antonio Gutiérrez-Barranquero
Sep 25, 2021·Journal, Genetic Engineering & Biotechnology·Shivani SinghK Narayanan Rajnish

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Methods Mentioned

BETA
PCR

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