Pseudomonas aeruginosa porin OprF: properties of the channel.

The Journal of Biological Chemistry
Ekaterina M NestorovichSergey M Bezrukov

Abstract

Using ion channel reconstitution in planar lipid bilayers, we examined the channel-forming activity of subfractions of Pseudomonas aeruginosa OprF, which was shown to exist in two different conformations: a minority single domain conformer and a majority two-domain conformer (Sugawara, E., Nestorovich, E. M., Bezrukov, S. M., and Nikaido, H. (2006) J. Biol. Chem. 281, 16220-16229). With the fraction depleted for the single domain conformer, we were unable to detect formation of any channels with well defined conductance levels. With the unfractionated OprF, we saw only rare channel formation. However, with the single domain-enriched fraction of OprF, we observed regular insertion of channels with highly reproducible conductances. Single OprF channels demonstrate rich kinetic behavior exhibiting spontaneous transitions between several subconformations that differ in ionic conductance and radius measured in polymer exclusion experiments. Although we showed that the effective radius of the most conductive conformation exceeds that of the general outer membrane porin of Escherichia coli, OmpF, we also found that a single OprF channel mainly exists in weakly conductive subconformations and switches to the fully open state for a shor...Continue Reading

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Citations

May 10, 2011·Biochemistry·Belete R ChenekeLiviu Movileanu
Oct 13, 2012·Chemical Reviews·Ekaterina M Nestorovich, Sergey M Bezrukov
Nov 11, 2008·Nature Reviews. Microbiology·Jean-Marie PagèsMathias Winterhalter
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Sep 23, 2006·The Journal of Biological Chemistry·Tatiana K RostovtsevaSergey M Bezrukov
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Mar 27, 2020·Frontiers in Bioengineering and Biotechnology·Ignacio Poblete-CastroJosé Manuel Borrero-de Acuña
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May 4, 2021·Frontiers in Cellular and Infection Microbiology·R Frèdi LangendonkJoanne L Fothergill
Jan 15, 2022·Proteomics·Wenxing Liu, Ekaterina M Nestorovich

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