Pseudomonas aeruginosa relA contributes to virulence in Drosophila melanogaster

Infection and Immunity
David L EricksonDouglas G Storey

Abstract

The stringent response is a mechanism by which bacteria adapt to nutritional deficiencies through the production of the guanine nucleotides ppGpp and pppGpp, produced by the RelA enzyme. We investigated the role of the relA gene in the ability of an extracellular pathogen, Pseudomonas aeruginosa, to cause infection. Strains lacking the relA gene were created from the prototypical laboratory strain PAO1 as well as the mucoid cystic fibrosis isolate 6106, which lacks functional quorum-sensing systems. The absence of relA abolished the production of ppGpp and pppGpp under conditions of amino acid starvation. We found that strains lacking relA exhibited reduced virulence in a D. melanogaster feeding assay. In conditions of low magnesium, the relA gene enhanced production of the cell-cell signal N-[3-oxododecanoyl]-l-homoserine lactone, whereas relA reduced the production of the 2-heptyl-3-hydroxy-4-quinolone signal during serine hydroxamate induction of the stringent response. In the relA mutant, alterations in the Pseudomonas quinolone system pathways seemed to increase the production of pyocyanin and decrease the production of elastase. Deletion of relA also resulted in reduced levels of the RpoS sigma factor. These results sugge...Continue Reading

References

Jan 1, 1979·Annual Review of Genetics·J A Gallant
Jun 1, 1987·Journal of Bacteriology·P A SchadB H Iglewski
Jan 1, 1983·Molecular & General Genetics : MGG·M HeckerF Mach
Sep 26, 1995·Proceedings of the National Academy of Sciences of the United States of America·M K WinsonB W Bycroft
Jul 3, 1995·Proceedings of the National Academy of Sciences of the United States of America·U A Ochsner, J Reiser
Feb 28, 1995·Proceedings of the National Academy of Sciences of the United States of America·J P PearsonE P Greenberg
Jan 4, 1994·Proceedings of the National Academy of Sciences of the United States of America·J P PearsonE P Greenberg
May 31, 1996·Journal of Molecular Biology·K D Murray, H Bremer
Aug 14, 1999·Molecular Microbiology·B K Hammer, M S Swanson
Sep 29, 1999·Proceedings of the National Academy of Sciences of the United States of America·E C PesciB H Iglewski
Apr 27, 2000·Journal of Bacteriology·S L McKnightE C Pesci
Jul 14, 2000·Journal of Bacteriology·M WhiteleyE P Greenberg
Aug 10, 2000·Proceedings of the National Academy of Sciences of the United States of America·M H RashidA Kornberg
Sep 6, 2000·Molecular Microbiology·E W Crawford, L J Shimkets
Feb 7, 2001·Journal of Bacteriology·D A D'ArgenioC Manoil
Feb 28, 2001·Proceedings of the National Academy of Sciences of the United States of America·S A ChuganiE P Greenberg
Jul 17, 2001·Molecular Microbiology·I KuharB J Jordi
Sep 7, 2001·Annual Review of Microbiology·M B Miller, B L Bassler
Sep 27, 2001·Proceedings of the National Academy of Sciences of the United States of America·M W CalfeeE C Pesci
Jan 16, 2002·Journal of Bacteriology·Clare M TaylorIan S Roberts
Feb 2, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Lynne R UsherMoira K B Whyte
Apr 23, 2002·Molecular Microbiology·Brian K HammerMichele S Swanson
Jul 27, 2002·Journal of Bacteriology·Larissa BrownMichael Cashel
Oct 24, 2002·FEMS Microbiology Letters·David N CollierEverett C Pesci
Feb 26, 2003·Proceedings of the National Academy of Sciences of the United States of America·Tina GuinaSamuel I Miller
Mar 20, 2003·Journal of Bacteriology·Victoria E WagnerBarbara H Iglewski

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Citations

Mar 16, 2007·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Paul WilliamsMiguel Cámara
Sep 28, 2010·Photochemistry and Photobiology·Cristina S CostaRamón A Pizarro
Nov 3, 2010·Infection and Immunity·Christina O IgboinEugene J Leys
Sep 9, 2008·Journal of Bacteriology·Nelli BoesMax Schobert
Jul 21, 2005·Journal of Bacteriology·Martine MorisJan Michiels
May 29, 2010·Microbiology and Molecular Biology Reviews : MMBR·Zachary D DalebrouxMichele S Swanson
Feb 18, 2011·Canadian Journal of Microbiology·Christopher D Sibley, Michael G Surette
Aug 16, 2008·The Journal of Clinical Investigation·David A StoltzJoseph Zabner
Mar 20, 2014·PloS One·Beny SpiraLuiz Gustavo de Almeida
Oct 31, 2009·PLoS Pathogens·James C CharitySimon L Dove
Mar 1, 2012·Journal of Oral Microbiology·Christina O IgboinEugene J Leys
Dec 31, 2014·International Journal of Molecular Sciences·Shaileja Chopra, John Reader
Apr 3, 2007·Developmental and Comparative Immunology·Stephanie M Brandt, David S Schneider
Apr 25, 2009·Journal of Cellular Physiology·Jun Wu, Jianping Xie
Aug 22, 2009·Journal of Evolutionary Biology·Jadene A YoungAneil F Agrawal
Nov 29, 2007·Cellular Microbiology·Laeticia AlibaudPierre Cosson
Dec 3, 2008·Cellular Microbiology·Cristin D BerkeyPaula I Watnick
Dec 2, 2008·Molecular Microbiology·Zachary D DalebrouxMichele S Swanson
Feb 6, 2008·Cellular Microbiology·Malin VonkavaaraSvenja Stöven
Sep 22, 2007·Cellular Microbiology·Mimi M Shirasu-Hiza, David S Schneider
Dec 24, 2014·Environmental Microbiology Reports·Pornsri CharoenpanichMatthew McIntosh
May 16, 2006·Microbes and Infection·Marion S Dorer, Ralph R Isberg
Dec 14, 2005·Trends in Microbiology·Kristien BraekenJan Michiels

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