Pseudomonas aeruginosa stimulates phosphorylation of the airway epithelial membrane glycoprotein Muc1 and activates MAP kinase

American Journal of Physiology. Lung Cellular and Molecular Physiology
Erik P LillehojK Chul Kim

Abstract

We reported previously that Muc1 on the surface of epithelial cells was a receptor for Pseudomonas aeruginosa (Lillehoj EP, Kim BT, and Kim KC. Am J Physiol Lung Cell Mol Physiol 282: L751-L756, 2002). Other studies showed that the Muc1 cytoplasmic tail (CT) contains multiple phosphorylation sites, some of which are phosphorylated constitutively and associated with signaling proteins. However, the relationship between extracellular P. aeruginosa binding and intracellular signaling is unknown. To investigate the signaling mechanism of Muc1, this study examined phosphorylation of its CT and activation of the extracellular signal-regulated kinase (ERK) in response to stimulation by P. aeruginosa or purified flagellin. Our results showed 1) the Muc1 CT was phosphorylated constitutively on serine and tyrosine, 2) serine phosphorylation was stimulated by bacterial cells or flagellin, and 3) binding of P. aeruginosa or flagellin to Muc1 induced phosphorylation of ERK. These results are the first to demonstrate Muc1 CT phosphorylation and ERK activation in response to a clinically important airway pathogen.

References

May 29, 1992·Biochemical and Biophysical Research Communications·L PembertonS J Gendler
Jan 1, 1983·Methods in Enzymology·J A CooperT Hunter
May 1, 1994·Infection and Immunity·P J BrettD E Woods
Aug 1, 1996·American Journal of Respiratory Cell and Molecular Biology·H ParkK C Kim
May 9, 1997·The Journal of Biological Chemistry·M YamamotoD Kufe
Jan 10, 1998·Infection and Immunity·M FeldmanA Prince
May 20, 1998·Proceedings of the National Academy of Sciences of the United States of America·J D LiC Basbaum
Jul 2, 1999·International Journal of Cancer. Journal International Du Cancer·M HartmanD H Wreschner
Mar 11, 2000·American Journal of Physiology. Lung Cellular and Molecular Physiology·D MeerzamanK C Kim
Aug 5, 2000·International Journal of Cancer. Journal International Du Cancer·R J Quin, M A McGuckin
Jan 3, 2001·American Journal of Physiology. Lung Cellular and Molecular Physiology·E P LillehojK C Kim
Feb 22, 2001·Biochemical and Biophysical Research Communications·Y Li, D Kufe
May 9, 2001·Biochemical and Biophysical Research Communications·S ParryA Harris
Jun 19, 2001·American Journal of Physiology. Lung Cellular and Molecular Physiology·D MeerzamanK C Kim
Sep 8, 2001·Journal of Mammary Gland Biology and Neoplasia·S J Gendler
Oct 12, 2001·Apoptosis : an International Journal on Programmed Cell Death·H GrassméE Gulbins
Mar 7, 2002·American Journal of Physiology. Lung Cellular and Molecular Physiology·Erik P LillehojK Chul Kim
Apr 5, 2002·Clinical Microbiology Reviews·Jeffrey B LyczakGerald B Pier
Aug 30, 2002·The Journal of Biological Chemistry·Laurence LévyMarie Annick Buendia
Dec 13, 2002·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·A O AzghaniG J Bhat
Jan 1, 2003·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Kermit L CarrawayCoralie A Carothers Carraway
Jan 4, 2003·Archives of Pharmacal Research·Erik R Lillehoj, K Chul Kim
May 17, 2003·Cancer Biology & Therapy·Yongqing LiDonald Kufe
Jul 2, 2003·The Journal of Biological Chemistry·Yunfei WenMichael A Hollingsworth
Aug 2, 2003·Biochemical and Biophysical Research Communications·Erik P LillehojK Chul Kim
Oct 3, 2003·Biochemical and Biophysical Research Communications·Honghe WangK Chul Kim

❮ Previous
Next ❯

Citations

May 31, 2012·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Tsuyoshi UmeharaKwang Chul Kim
Jun 14, 2005·Trends in Microbiology·Gee W LauBradley E Britigan
Aug 21, 2010·Respirology : Official Journal of the Asian Pacific Society of Respirology·Bryan J WilliamsTimothy S Blackwell
Sep 14, 2007·Annual Review of Physiology·Christine L Hattrup, Sandra J Gendler
Jan 28, 2006·American Journal of Respiratory Cell and Molecular Biology·Alice Prince
Dec 15, 2007·American Journal of Respiratory Cell and Molecular Biology·Keiko UenoK Chul Kim
Jul 12, 2008·American Journal of Respiratory Cell and Molecular Biology·K Chul Kim, Erik P Lillehoj
May 8, 2010·American Journal of Respiratory Cell and Molecular Biology·Seongwon ChoiKwang Chul Kim
Jul 21, 2007·The Journal of Clinical Investigation·Julie L McAuleyMichael A McGuckin
Jul 19, 2006·Breast Cancer Research : BCR·Christine L Hattrup, Sandra J Gendler
Dec 29, 2009·Frontiers in Bioscience (Elite Edition)·Kosuke KatoKwang Chul Kim
Dec 27, 2011·Pulmonary Pharmacology & Therapeutics·Kwang Chul Kim
Jan 12, 2011·Biochemical and Biophysical Research Communications·Akinori HisatsuneHiroshi Katsuki
Mar 13, 2010·Experimental Eye Research·Bharathi Govindarajan, Ilene K Gipson
Aug 18, 2009·Biochemical and Biophysical Research Communications·Akinori HisatsuneKwang Chul Kim
Feb 10, 2009·Veterinary Immunology and Immunopathology·Ezequiel LacunzaMaría V Croce
Aug 28, 2009·Journal of Cellular Biochemistry·Joanne JulianDaniel D Carson
Jan 6, 2009·Journal of Applied Microbiology·A NejidatZ Ronen
Jan 15, 2005·Biochemical and Biophysical Research Communications·Susanne KirschnekErich Gulbins
Sep 11, 2004·Biochemical and Biophysical Research Communications·Honghe WangK Chul Kim
Aug 15, 2006·Trends in Cell Biology·Pankaj K Singh, Michael A Hollingsworth
Jan 15, 2014·Biochimica Et Biophysica Acta·Kamiya Mehla, Pankaj K Singh
May 26, 2007·Biochimica Et Biophysica Acta·Erik P LillehojK Chul Kim
May 8, 2016·The Ocular Surface·Anna F Ablamowicz, Jason J Nichols
Mar 23, 2017·Mucosal Immunology·J L McAuleyL E Brown
Dec 1, 2017·Journal of Clinical Medicine·Kosuke KatoKwang Chul Kim
Jan 5, 2017·Journal of Innate Immunity·Jos P M van Putten, Karin Strijbis
Sep 1, 2017·Immunological Reviews·Jeremy J Barr
Nov 28, 2018·Investigative Ophthalmology & Visual Science·Yuichi Uchino
Dec 17, 2008·Mucosal Immunology·S K LindenM A McGuckin
Jun 19, 2007·American Journal of Physiology. Lung Cellular and Molecular Physiology·Takeshi KogaK Chul Kim
Aug 8, 2019·The Biochemical Journal·Hannah SchneiderGunnar C Hansson
Feb 6, 2020·Cellular Microbiology·Yueqin LiuJoseph A Kovacs
Apr 26, 2005·American Journal of Physiology. Lung Cellular and Molecular Physiology·Ippei KuwaharaK Chul Kim
Jun 18, 2017·Current Opinion in Nephrology and Hypertension·Mohammad M Al-BatainehRebecca P Hughey
Jun 21, 2013·American Journal of Physiology. Cell Physiology·Thaher PelaseyedGunnar C Hansson

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.