Pseudomonas aeruginosa type IV minor pilins and PilY1 regulate virulence by modulating FimS-AlgR activity

PLoS Pathogens
Victoria A MarkoLori L Burrows

Abstract

Type IV pili are expressed by a wide range of prokaryotes, including the opportunistic pathogen Pseudomonas aeruginosa. These flexible fibres mediate twitching motility, biofilm maturation, surface adhesion, and virulence. The pilus is composed mainly of major pilin subunits while the low abundance minor pilins FimU-PilVWXE and the putative adhesin PilY1 prime pilus assembly and are proposed to form the pilus tip. The minor pilins and PilY1 are encoded in an operon that is positively regulated by the FimS-AlgR two-component system. Independent of pilus assembly, PilY1 was proposed to be a mechanosensory component that-in conjunction with minor pilins-triggers up-regulation of acute virulence phenotypes upon surface attachment. Here, we investigated the link between the minor pilins/PilY1 and virulence. pilW, pilX, and pilY1 mutants had reduced virulence towards Caenorhabditis elegans relative to wild type or a major pilin mutant, implying a role in pathogenicity that is independent of pilus assembly. We hypothesized that loss of specific minor pilins relieves feedback inhibition on FimS-AlgR, increasing transcription of the AlgR regulon and delaying C. elegans killing. Reporter assays confirmed that FimS-AlgR were required for ...Continue Reading

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Citations

Sep 22, 2019·Applied and Environmental Microbiology·Tao FengYan Wang
Aug 5, 2020·Journal of Bacteriology·Ashley R CrossJoanna B Goldberg
Nov 27, 2019·Applied Microbiology and Biotechnology·Fazlurrahman KhanYoung-Mog Kim
Dec 1, 2019·Medical Microbiology and Immunology·Theis JacobsenMichael Nilges
Oct 9, 2020·Nature Communications·Anke Treuner-LangeLotte Søgaard-Andersen
Oct 21, 2020·Journal of Bacteriology·Katherine J Graham, Lori L Burrows
Sep 4, 2020·Expert Opinion on Drug Discovery·Xiaolong ShaoXin Deng
Apr 4, 2021·International Journal of Molecular Sciences·Irene Jurado-MartínSiobhán McClean
Feb 10, 2019·Microbiology Spectrum·Sandra MuschiolBirgitta Henriques-Normark
Nov 18, 2021·FEMS Microbiology Reviews·Courtney K EllisonYves V Brun

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Methods Mentioned

BETA
two-hybrid
motility
motility assays

Software Mentioned

ImageJ
GraphPad Prism

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