Pseudomonas aeruginosa, under DNA replication inhibition, tends to form biofilms via Arr

Research in Microbiology
Hideo GotohTao Weitao

Abstract

Bacteria infecting eukaryotic hosts often encounter therapeutic antimicrobial and DNA damaging agents and respond by forming biofilms. While mechanisms of biofilm response are incompletely understood, they seem to involve bacterial second messenger bis-(3'-5')-cyclic dimeric guanosine monophosphate (c-di-GMP) signaling. We hypothesized that DNA replication inhibition induces bacterial biofilm formation via c-di-GMP signaling. Evidently, we found that Pseudomonas aeruginosa mounted a biofilm response to the subinhibitory DNA replication inhibitors hydroxyurea and nalidixic acid, but planktonic proliferation was inhibited. The biofilm response was suppressed either genetically by mutations causing planktonic resistance or biochemically by reversal of replication inhibition. Biofilms were induced by a mechanism of stimulated adhesion of planktonic filaments having impaired DNA replication, as examined under fluorescence microscopy. Induction was suppressed by either inhibition or mutation of Arr-a c-di-GMP phosphodiesterase. These results suggest that P. aeruginosa, under DNA replication stress, tends to form biofilms via Arr. The profound implications of the SOS response, planktonic-sessile and bacteria-cancer relationships are d...Continue Reading

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Citations

Aug 13, 2013·Colloids and Surfaces. B, Biointerfaces·Amit Kumar, Yen-Peng Ting
Feb 26, 2010·Infectious Agents and Cancer·Shatha F Dallo, Tao Weitao
Mar 30, 2013·Biotechnology Letters·Xiao ZhangHonglei Liu
Jul 7, 2010·Biofouling·H GotohT Weitao
Feb 19, 2010·Applied Microbiology and Biotechnology·Paolo LandiniReindert Nijland
Dec 19, 2008·Medical Hypotheses·Tao Weitao
Nov 11, 2008·Research in Microbiology·Manuel Espinosa-Urgel
Mar 27, 2012·TheScientificWorldJournal·Reshma MarediaTao Weitao
Apr 4, 2018·Journal of Bacteriology·Nicolas L FernandezChristopher M Waters
Aug 27, 2014·Infection and Immunity·Sahar A Alshalchi, Gregory G Anderson
Mar 20, 2010·Advances in Skin & Wound Care·Shatha F Dallo, Tao Weitao
Nov 6, 2020·Prostaglandins & Other Lipid Mediators·J M ThorntonK Yin
Feb 11, 2021·International Journal of Molecular Sciences·Gianmarco MangiaterraFrancesca Biavasco
Dec 23, 2020·The Journal of Antibiotics·Derek C K Chan, Lori L Burrows
Nov 7, 2021·Proceedings of the National Academy of Sciences of the United States of America·Mario K C KrespachAxel A Brakhage

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