Pseudomonas chlororaphis Produces Multiple R-Tailocin Particles That Broaden the Killing Spectrum and Contribute to Persistence in Rhizosphere Communities

Applied and Environmental Microbiology
Robert J DoroskyElizabeth A Pierson

Abstract

R-tailocins are high-molecular-weight bacteriocins resembling bacteriophage tails. Pseudomonas chlororaphis 30-84 is a plant growth-promoting rhizobacterial (PGPR) strain that produces two distinct R-tailocin particles with different killing spectra. The two R-tailocins have different evolutionary histories but are released by the same lysis cassette. A previous study showed that both tailocins are important for pairwise competition with susceptible rhizosphere-colonizing strains; however, the broader role of tailocins in competition with the native rhizosphere microbiome was not tested. Genomic analysis of the P. chlororaphis 30-84 R-tailocin gene cluster uncovered the presence of three tail fiber genes in the tailocin 2 genetic module that could potentially result in tailocin 2 particles having different tail fibers and thus a wider killing spectrum. In this study, the tail fibers were found to incorporate onto different tailocin 2 particles, each with a distinct killing spectrum. A loss of production of one or both tailocins resulted in decreased P. chlororaphis 30-84 persistence within the wheat rhizosphere when in competition with the native microflora but not bulk soil. The capacity to produce three different versions of ...Continue Reading

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Citations

Feb 12, 2020·Journal of Medical Microbiology·Anne J Anderson, Young Cheol Kim
Feb 21, 2019·Biochemical Society Transactions·Dorien DamsYves Briers
Oct 13, 2020·Frontiers in Microbiology·William M RooneyDaniel Walker
Dec 12, 2020·Computational and Structural Biotechnology Journal·Antoine Zboralski, Martin Filion

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