Pseudomonas chlororaphis Produces Two Distinct R-Tailocins That Contribute to Bacterial Competition in Biofilms and on Roots

Applied and Environmental Microbiology
Robert J DoroskyElizabeth A Pierson

Abstract

R-type tailocins are high-molecular-weight bacteriocins that resemble bacteriophage tails and are encoded within the genomes of many Pseudomonas species. In this study, analysis of the P. chlororaphis 30-84 R-tailocin gene cluster revealed that it contains the structural components to produce two R-tailocins of different ancestral origins. Two distinct R-tailocin populations differing in length were observed in UV-induced lysates of P. chlororaphis 30-84 via transmission electron microscopy. Mutants defective in the production of one or both R-tailocins demonstrated that the killing spectrum of each tailocin is limited to Pseudomonas species. The spectra of pseudomonads killed by the two R-tailocins differed, although a few Pseudomonas species were either killed by or insusceptible to both tailocins. Tailocin release was disrupted by deletion of the holin gene within the tailocin gene cluster, demonstrating that the lysis cassette is required for the release of both R-tailocins. The loss of functional tailocin production reduced the ability of P. chlororaphis 30-84 to compete with an R-tailocin-sensitive strain within biofilms and rhizosphere communities. Our study demonstrates that Pseudomonas species can produce more than one...Continue Reading

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Citations

Jun 15, 2019·International Journal of Environmental Research and Public Health·Yuman Zhou, Xiaorong Gao
Feb 12, 2020·Journal of Medical Microbiology·Anne J Anderson, Young Cheol Kim
Mar 22, 2018·MBio·Maarten G K GhequireRené De Mot
Nov 30, 2018·Frontiers in Microbiology·Maarten G K GhequireRené De Mot
Oct 13, 2020·Frontiers in Microbiology·William M RooneyDaniel Walker
Dec 12, 2020·Computational and Structural Biotechnology Journal·Antoine Zboralski, Martin Filion
Nov 8, 2019·Journal of Biotechnology·Maricruz FernandezSonia Fischer
Nov 5, 2021·Environmental Microbiology·Kateřina SnopkováDavid Šmajs

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