Pseudomonas fluorescens and Trichoderma asperellum Enhance Expression of Gα Subunits of the Pea Heterotrimeric G-protein during Erysiphe pisi Infection

Frontiers in Plant Science
Jai S PatelMushtaq Ahmed

Abstract

We investigated the transcript accumulation patterns of all three subunits of heterotrimeric G-proteins (Gα1 and 2, Gβ, and Gγ) in pea under stimulation of two soil-inhabiting rhizosphere microbes Pseudomonas fluorescens OKC and Trichoderma asperellum T42. The microbes were either applied individually or co-inoculated and the transcript accumulation patterns were also investigated after challenging the same plants with a fungal biotrophic pathogen Erysiphe pisi. We observed that mostly the transcripts of Gα 1 and 2 subunits were accumulated when the plants were treated with the microbes (OKC and T42) either individually or co-inoculated. However, transcript accumulations of Gα subunits were highest in the T42 treatment particularly under the challenge of the biotroph. Transcript accumulations of the other two subunits Gβ and Gγ were either basal or even lower than the basal level. There was an indication for involvement of JA-mediated pathway in the same situations as activation of LOX1 and COI1 were relatively enhanced in the microbe co-inoculated treatments. Non-increment of SA content as well as transcripts of SA-dependent PR1 suggested non-activation of the SA-mediated signal transduction in the interaction of pea with E. p...Continue Reading

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Citations

May 21, 2016·Applied Microbiology and Biotechnology·Indu KumariYusuf Akhter
Jul 13, 2017·Journal of Agricultural and Food Chemistry·Sudheer K YadavBirinchi K Sarma
Oct 23, 2019·Journal of the Science of Food and Agriculture·Zhirong WangJianquan Kan
May 2, 2020·3 Biotech·Jai Singh PatelBirinchi Kumar Sarma

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Datasets Mentioned

BETA
JN128894
JN128891

Methods Mentioned

BETA
PCR

Software Mentioned

Primer
Shimadzu Class VP

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