Pseudomonas orientalis F9: A Potent Antagonist against Phytopathogens with Phytotoxic Effect in the Apple Flower

Frontiers in Microbiology
Veronika ZengererCosima Pelludat

Abstract

In light of public concerns over the use of pesticides and antibiotics in plant protection and the subsequent selection for spread of resistant bacteria in the environment, it is inevitable to broaden our knowledge about viable alternatives, such as natural antagonists and their mode of action. The genus Pseudomonas is known for its metabolic versatility and genetic plasticity, encompassing pathogens as well as antagonists. We characterized strain Pseudomonas orientalis F9, an isolate from apple flowers in a Swiss orchard, and determined its antagonistic activity against several phytopathogenic bacteria, in particular Erwinia amylovora, the causal agent of fire blight. P. orientalis F9 displayed antagonistic activity against a broad suite of phytopathogenic bacteria in the in vitro tests. The promising results from this analysis led to an ex vivo assay with E. amylovora CFBP1430Rif and P. orientalis F9 infected detached apple flowers. F9 diminished the fire blight pathogen in the flowers but also revealed phytotoxic traits. The experimental results were discussed in light of the complete genome sequence of F9, which revealed the strain to carry phenazine genes. Phenazines are known to contribute to antagonistic activity of bact...Continue Reading

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Citations

Mar 29, 2020·Microorganisms·Moritz MiebachMitja N P Remus-Emsermann
Sep 24, 2020·FEMS Microbiology Ecology·Guillaume ChesneauMatthieu Barret

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Datasets Mentioned

BETA
CP018049
PRJNA353169
AY061859.1

Methods Mentioned

BETA
biotyping
Assay

Software Mentioned

PILER
SMRT
MUSCLE
Prism
Ori
RAST Server
Finder
RAxML
CRISPRFinder
SPAdes

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