Pseudomonas putida KT2440 responds specifically to chlorophenoxy herbicides and their initial metabolites

Proteomics
Dirk BenndorfHauke Harms

Abstract

Pseudomonas putida KT2440 is often used as a model to investigate toxicity mechanisms and adaptation to hazardous chemicals in bacteria. The objective of this paper was to test the impact of the chlorophenoxy herbicides 2,4-dichlorophenoxyacetic acid (2,4-D) and 2-(2,4-dichlorophenoxy)propanoic acid (DCPP) and their metabolites 2,4-dichlorophenol (DCP) and 3,5-dichlorocatechol (DCC), on protein expression patterns and physiological parameters. Both approaches showed that DCC has a different mode of action and induces different responses than DCPP, 2,4-D and DCP. DCC was the most toxic compound and was active as an uncoupler of oxidative phosphorylation. It repressed the synthesis of ferric uptake regulator (Fur)-dependent proteins, e.g. fumarase C and L-ornithine N5-oxygenase, which are involved in oxidative stress response and iron uptake. DCPP, 2,4-D and DCP were less toxic than DCC. They disturbed oxidative phosphorylation to a lesser extent by a yet unknown mechanism. Furthermore, they repressed enzymes of energy-consuming biosynthetic pathways and induced membrane transporters for organic substrates. A TolC homologue component of multidrug resistance transporters was found to be induced, which is probably involved in the r...Continue Reading

References

Apr 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·A R Proctor, I P Crawford
Jan 1, 1991·Clinical Microbiology Reviews·P H Gilligan
Sep 15, 2000·Biological Chemistry·M C Ganoza, H Aoki
Oct 6, 2001·Current Opinion in Microbiology·K Poole
Jul 27, 2002·Annual Review of Microbiology·Robert E W Hancock, Fiona S L Brinkman
Jul 27, 2002·Annual Review of Microbiology·Juan L RamosAna Segura
Jan 22, 2003·Environmental Microbiology·José Ignacio JiménezEduardo Díaz
Dec 4, 2003·Environmental Microbiology·John E HallsworthKenneth N Timmis
Dec 17, 2003·Archives of Environmental Contamination and Toxicology·N LoffhagenW Babel
Nov 25, 2004·Environmental Microbiology·V A P Martins Dos SantosK N Timmis
May 1, 1997·Applied and Environmental Microbiology·P Vercellone-Smith, D S Herson

❮ Previous
Next ❯

Citations

Sep 22, 2007·Omics : a Journal of Integrative Biology·Seung Il KimHyung-Yeel Kahng
Sep 8, 2010·Journal of Environmental Science and Health. Part. B, Pesticides, Food Contaminants, and Agricultural Wastes·Ali FanousFritz Jacob
Jun 25, 2014·Applied Microbiology and Biotechnology·Jisun Kim, Woojun Park
Feb 22, 2012·Applied Microbiology and Biotechnology·Ignacio Poblete-CastroChristoph Wittmann
Apr 22, 2009·Proteomics·Roberto A Bobadilla FazziniVitor A P Martins dos Santos
Apr 22, 2010·Environmental Microbiology·Roberto A Bobadilla FazziniVitor A P Martins Dos Santos
Jan 25, 2011·Microbial Biotechnology·Prashanth BhagannaJohn E Hallsworth
Nov 13, 2010·Journal of Applied Microbiology·N JehmlichJ Seifert
Nov 28, 2015·Current Genetics·Prashanth BhagannaJohn E Hallsworth
Oct 26, 2006·The Journal of Antimicrobial Chemotherapy·Nick G ColdhamMartin J Woodward
Nov 29, 2007·The ISME Journal·Dirk BenndorfMartin von Bergen
Mar 25, 2017·Nanomaterials·Tiago FernandesAna L Daniel-da-Silva
Aug 10, 2020·Environmental Science and Pollution Research International·Karen MagnoliCarla Lorena Barberis
Nov 14, 2017·Biotechnology and Bioengineering·Patricia CaleroAlex T Nielsen

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacterial Transport Proteins

Bacterial transport proteins facilitate active and passive transport of small molecules and solutes across the bacterial membrane. Here is the latest research.

Bacterial Transport Proteins (ASM)

Bacterial transport proteins facilitate active and passive transport of small molecules and solutes across the bacterial membrane. Here is the latest research.