Pseudomonas putida KT2440 Strain Metabolizes Glucose through a Cycle Formed by Enzymes of the Entner-Doudoroff, Embden-Meyerhof-Parnas, and Pentose Phosphate Pathways.

The Journal of Biological Chemistry
Pablo I NikelVíctor de Lorenzo

Abstract

The soil bacterium Pseudomonas putida KT2440 lacks a functional Embden-Meyerhof-Parnas (EMP) pathway, and glycolysis is known to proceed almost exclusively through the Entner-Doudoroff (ED) route. To investigate the raison d'être of this metabolic arrangement, the distribution of periplasmic and cytoplasmic carbon fluxes was studied in glucose cultures of this bacterium by using (13)C-labeled substrates, combined with quantitative physiology experiments, metabolite quantification, and in vitro enzymatic assays under both saturating and non-saturating, quasi in vivo conditions. Metabolic flux analysis demonstrated that 90% of the consumed sugar was converted into gluconate, entering central carbon metabolism as 6-phosphogluconate and further channeled into the ED pathway. Remarkably, about 10% of the triose phosphates were found to be recycled back to form hexose phosphates. This set of reactions merges activities belonging to the ED, the EMP (operating in a gluconeogenic fashion), and the pentose phosphate pathways to form an unforeseen metabolic architecture (EDEMP cycle). Determination of the NADPH balance revealed that the default metabolic state of P. putida KT2440 is characterized by a slight catabolic overproduction of re...Continue Reading

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Citations

Jul 21, 2016·Microbial Biotechnology·Antoine Danchin, Gang Fang
Sep 24, 2016·Current Opinion in Biotechnology·Esteban Martínez-García, Víctor de Lorenzo
Feb 26, 2016·Environmental Microbiology·Eugeni BeldaClaudine Médigue
Mar 16, 2017·Biotechnology Journal·Sarah Beate Hintermayer, Dirk Weuster-Botz
Dec 15, 2016·Frontiers in Bioengineering and Biotechnology·Shiqin YuJens O Krömer
Oct 24, 2017·Biotechnology Journal·Tomás AparicioEsteban Martínez-García
Jul 19, 2018·Molecular Microbiology·Max Chavarría, Víctor de Lorenzo
Aug 9, 2018·Chembiochem : a European Journal of Chemical Biology·Justin A ShapiroWilliam M Wuest
Oct 27, 2015·Applied and Environmental Microbiology·Manuel S GodoyM Julia Pettinari
Sep 14, 2018·Environmental Microbiology·Danilo Pérez-PantojaVíctor de Lorenzo
Apr 9, 2019·Microbial Biotechnology·Ángeles Hueso-GilVíctor de Lorenzo
Jun 4, 2019·Proceedings of the National Academy of Sciences of the United States of America·Reto S WijkerMichelle Phan
Nov 27, 2019·Cellular and Molecular Life Sciences : CMLS·Heejoon ParkRoss P Carlson
Feb 12, 2020·Microbial Biotechnology·Christos BatianisVitor A P Martins Dos Santos
Apr 9, 2020·Microbial Biotechnology·Andreas AnkenbauerRalf Takors
Apr 30, 2020·Extremophiles : Life Under Extreme Conditions·Quynh DangThuChangWoo Lee
Apr 11, 2020·Chembiochem : a European Journal of Chemical Biology·Manuel Nieto-Domínguez, Pablo I Nikel
Nov 3, 2017·Microbial Biotechnology·Deirdre E RicaurteTomás Aparicio
Aug 14, 2020·Applied Microbiology and Biotechnology·Anna WeimerChristoph Wittmann
Jun 11, 2020·Environmental Microbiology·Ángeles Hueso-GilVíctor de Lorenzo
Sep 11, 2019·Microbial Cell Factories·Sofía Hernandez-ArranzManuel Rodriguez-Concepcion
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Aug 16, 2018·Applied Biochemistry and Biotechnology·Wenjing SunXianghui Qi

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