Pseudomonas viridiflava, a multi host plant pathogen with significant genetic variation at the molecular level.

PloS One
Panagiotis F SarrisDimitrios E Goumas

Abstract

The pectinolytic species Pseudomonas viridiflava has a wide host range among plants, causing foliar and stem necrotic lesions and basal stem and root rots. However, little is known about the molecular evolution of this species. In this study we investigated the intraspecies genetic variation of P. viridiflava amongst local (Cretan), as well as international isolates of the pathogen. The genetic and phenotypic variability were investigated by molecular fingerprinting (rep-PCR) and partial sequencing of three housekeeping genes (gyrB, rpoD and rpoB), and by biochemical and pathogenicity profiling. The biochemical tests and pathogenicity profiling did not reveal any variability among the isolates studied. However, the molecular fingerprinting patterns and housekeeping gene sequences clearly differentiated them. In a broader phylogenetic comparison of housekeeping gene sequences deposited in GenBank, significant genetic variability at the molecular level was found between isolates of P. viridiflava originated from different host species as well as among isolates from the same host. Our results provide a basis for more comprehensive understanding of the biology, sources and shifts in genetic diversity and evolution of P. viridiflava...Continue Reading

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Citations

Feb 4, 2014·Microbiological Research·Nádia CastanheiraPaula Fareleira
May 2, 2017·PloS One·Barbara TressBianka S Schulz
Dec 21, 2017·BMC Genomics·Rong-Cai YangZhiqiu Hu
Mar 19, 2020·Frontiers in Plant Science·Andrea FirrincieliSharon Lafferty Doty
Apr 26, 2021·Doklady. Biochemistry and Biophysics·K K FursovaF A Brovko
May 13, 2021·Applied Microbiology and Biotechnology·Suhana ChattopadhyayAmy R Sapkota
Sep 1, 2021·Molecular Plant Pathology·Savana M Lipps, Deborah A Samac

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Methods Mentioned

BETA
Fluorescence
Electrophoresis
PCR

Software Mentioned

MEGA5
UPGMA
MEGA ( Molecular Evolutionary Genetics Analysis )
NTSYSpc
CLUSTALW

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