Pseudopod projection and cell spreading of passive leukocytes in response to fluid shear stress

Biophysical Journal
Mark F Coughlin, Geert W Schmid-Schönbein

Abstract

Recent evidence suggests that circulating leukocytes respond to physiological levels of fluid shear stress. This study was designed to examine the shear stress response of individual leukocytes adhering passively to a glass surface. Human leukocytes were exposed to a step fluid shear stress with amplitude between 0.2 and 4 dyn/cm(2) and duration between 1 and 20 min. The response of the cells was determined in the form of projected cell area measurements by high-resolution observation before, during, and after fluid shear application. All cells selected initially had a round morphology. After application of fluid shear many cells projected pseudopodia and spread on the glass surface. The number of leukocytes responding with pseudopod projection and the extent of cell spreading increased with increasing amplitude and duration of fluid shear stress. Pseudopod projection after exposure to a step fluid shear occurs following a delay that is insensitive to the shear stress amplitude and duration. Leukocytes that did not project pseudopodia and spread in response to low shear stress could be shown to respond to a second shear step of higher amplitude. The spreading response requires an intact actin network and activated myosin molecu...Continue Reading

References

Jun 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·B ArdmanD E Staunton
Feb 1, 1986·Biophysical Journal·D C TorneyG I Bell
Jun 1, 1984·Biophysical Journal·G I BellP Bongrand
Sep 1, 1995·Annals of Biomedical Engineering·H P Ting-BeallR M Hochmuth
Jun 1, 1996·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·K L OhashiG W Schmid-Schönbein
May 13, 1997·Proceedings of the National Academy of Sciences of the United States of America·F MoazzamG W Schmid-Schönbein
Jul 9, 1999·Medical & Biological Engineering & Computing·R S Rosenson-SchlossP V Moghe
May 24, 2000·Proceedings of the National Academy of Sciences of the United States of America·M S ShiveJ M Anderson
Nov 6, 2001·Molecular Biology of the Cell·S SeveauL M Pierini
Jun 8, 2002·Annals of Biomedical Engineering·Peter Marschel, Geert W Schmid-Schönbein
Sep 11, 2003·Journal of Cell Science·Emmanuel DécaveFranz Bruckert
Nov 19, 2003·Journal of Anatomy·Margaret M BirdA W Segal

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Citations

Jul 7, 2009·European Biophysics Journal : EBJ·Cécile CouzonClaude Verdier
Feb 23, 2008·Journal of Mathematical Biology·Claude VerdierPushpendra Singh
Mar 24, 2010·Biomechanics and Modeling in Mechanobiology·Liyue LiuJinfu Wang
Oct 22, 2005·Annals of Biomedical Engineering·Yutaka Komai, Geert W Schmid-Schönbein
Nov 17, 2007·Annals of Biomedical Engineering·Susan S Su, Geert W Schmid-Schönbein
Nov 21, 2008·Clinical and Experimental Pharmacology & Physiology·Scott I SimonUlrich Y Schaff
Jul 13, 2006·Annual Review of Biomedical Engineering·Geert W Schmid-Schönbein
Oct 3, 2006·Cell Biochemistry and Biophysics·Ilka Lorenzen-SchmidtJeffrey H Omens
Apr 5, 2012·The Journal of Cell Biology·Jacob RulloMyron I Cybulsky
Nov 10, 2009·Journal of Biomechanics·Jean-François GanghofferNacim Mefti
Dec 5, 2015·Biophysical Journal·Tenghu Wu, James J Feng
Oct 5, 2007·Journal of Immunological Methods·Dooyoung Lee, Michael R King
Oct 9, 2007·Biophysical Journal·Mark F CoughlinGeert W Schmid-Schönbein
Feb 25, 2006·Journal of Biomechanics·Roxana Chotard-GhodsniaAlain Duperray
Jul 28, 2015·Biochimica Et Biophysica Acta·Andrew E EkpenyongJochen Guck
Oct 16, 2012·Biophysical Journal·Amir H G Isfahani, Jonathan B Freund
Feb 28, 2013·Biophysical Journal·Marie-Pierre ValignatAnnemarie C Lellouch
Jul 10, 2012·Biophysical Journal·Michael J Mitchell, Michael R King
Apr 9, 2008·Biophysical Journal·Aleksandr RabodzeyBenoit Ladoux
Jun 20, 2015·PloS One·Anne D RocheleauMichael R King
Oct 16, 2016·The Journal of Cell Biology·Noah FineRobert Rottapel
Mar 8, 2018·Artificial Organs·Ina Laura PieperCatherine A Thornton
Apr 9, 2010·Cellular and Molecular Bioengineering·Susan S Su, Geert W Schmid-Schönbein
Nov 25, 2004·American Journal of Physiology. Cell Physiology·Ayako MakinoGeert W Schmid-Schönbein
Aug 27, 2005·Journal of Applied Physiology·Belinda Yap, Roger D Kamm
Apr 5, 2016·Cellular and Molecular Bioengineering·Anne D RocheleauMichael R King
May 21, 2010·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·Roland Bouffanais, Dick K P Yue
Sep 14, 2010·Wiley Interdisciplinary Reviews. Systems Biology and Medicine·Albert J KeungDavid V Schaffer
May 21, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Mia PhillipsonPaul Kubes
Aug 3, 2019·Cellular and Molecular Bioengineering·Christopher S LewisDavid W Schmidtke
Mar 31, 2021·Journal of Biomechanics·Hainsworth Y ShinGeert W Schmid-Schönbein
Feb 9, 2018·Chemical Reviews·Deborah HuberGovind V Kaigala

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