PMID: 2499870May 25, 1989Paper

Pseudorevertants of a lac promoter mutation reveal overlapping nascent promoters

Nucleic Acids Research
R KarlsW S Reznikoff

Abstract

Four pseudorevertants of a -10 region lacP mutation were isolated. Three of these mutations were found to activate nascent promoters. These mutations were: a -2 G/C----A/T change (-2A) promoting transcription at position +11, a +1 A/T----T/A change (+1T) promoting transcription initiation at position +13, and a +10 C/G----A/T change (+10A) promoting transcription initiation at a complex series of positions. The fourth mutation [a -12 T/A----A/T change (-12A)] promotes transcription initiation at -1. The promoters activated by mutations -12A, -2A and +1T resembled the canonical sigma 70 promoter sequences. The +10A promoter activity is also dependent upon the sigma 70 holoenzyme but can not be readily assigned to a specific promoter sequence.

References

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Sep 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·S W Artz, J R Broach
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Citations

Sep 5, 1991·Journal of Molecular Biology·V P Schulz, W S Reznikoff
May 4, 2004·Nature Structural & Molecular Biology·Bryce E NickelsAnn Hochschild
May 4, 2004·Nature Structural & Molecular Biology·Konstantin BrodolinHermann Heumann
Nov 20, 1991·Journal of Molecular Biology·P Rockwell, M E Gottesman
Sep 1, 1992·Molecular Microbiology·W S Reznikoff
Apr 20, 2018·Nature Communications·Avihu H YonaJeff Gore
Apr 1, 1996·Journal of Bacteriology·J A Leeds, R A Welch
Aug 1, 1995·Journal of Bacteriology·J E Flory, T J Donohue
Jul 1, 1991·Journal of Bacteriology·B J MacGregor, T J Donohue

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