PMID: 8602365Feb 1, 1996Paper

Psoralen crosslinking between human immunodeficiency virus type 1 RNA and primer tRNA3(Lys)

Nucleic Acids Research
E SkripkinC Ehresmann

Abstract

Initiation of reverse transcription is a crucial step of retroviral infection. In HIV-1, it involves hybridization of the 18 3'-terminal nucleotides of the primer tRNA3(Lys) to the primer binding site (PBS) of the viral RNA. Moreover, additional interactions between the two RNAs were recently evidenced [Isel et al. (1995) J. Mol. Biol. 247, 25269-25272]. To get further information on the topology of the viral RNA/tRNA3(Lys) complex, we used psoralen to induce RNA-RNA crosslinking. A defined intermolecular crosslinked complex was obtained. The crosslinked regions were characterized by RNase T1 digestion followed by bi-dimensional gel electrophoresis. The crosslinked residues (nucleotide mcm5S2U34 and U35 in the anticodon loop of tRNA3(Lys) and UCU154 in the viral RNA upstream of the PBS) were mapped using a retardation method coupled with random hydrolysis. The formation of this crosslink depends on the same elements that are required for the formation of the extended interactions between primer and template RNAs, i.e., the modified bases of the tRNA and a conserved A-rich loop located upstream of the PBS in the genomic RNA. Therefore, the present crosslinking data provide relevant information on the topology of the template/pri...Continue Reading

References

Sep 5, 1976·Journal of Molecular Biology·W A HaseltineD Baltimore
Sep 11, 1979·Nucleic Acids Research·H Donis-Keller
Oct 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·L A Kohlstaedt, T A Steitz
Jun 30, 1992·Biochemical and Biophysical Research Communications·M JiangL Kleiman
Feb 14, 1991·Biochemical and Biophysical Research Communications·L KleimanM A Parniak
Jan 24, 1985·Nature·L RatnerK Baumeister
Jan 1, 1988·Methods in Enzymology·S E Lipson, J E Hearst
May 24, 1994·Proceedings of the National Academy of Sciences of the United States of America·E SkripkinC Ehresmann
Feb 1, 1993·Molecular Biology Reports·D A Wassarman

❮ Previous
Next ❯

Citations

Dec 6, 2000·RNA·M R Hargittai, K Musier-Forsyth
Apr 28, 2000·European Journal of Biochemistry·F BoulméS Litvak
Apr 4, 1998·Nucleic Acids Research·C IselR Marquet
Nov 17, 2009·Nucleic Acids Research·Gaëlle MercenneRoland Marquet
Feb 12, 1998·Proceedings of the National Academy of Sciences of the United States of America·B Chan, K Musier-Forsyth
Jan 8, 1999·Journal of Molecular Biology·T ElgavishS C Harvey
Jan 1, 2010·Viruses·Catherine IselRoland Marquet
Aug 27, 2002·The Journal of Biological Chemistry·Valerie GoldschmidtRoland Marquet
Apr 15, 2000·The Journal of Biological Chemistry·J M LanchyR Marquet
May 16, 2000·The Journal of Biological Chemistry·N Beerens, B Berkhout
Aug 8, 1998·The Journal of Biological Chemistry·C LiangM A Wainberg
Jan 1, 1999·Hematology·R S TaichmanS G Emerson

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.