Abstract
Psoralen has potential hepatotoxicity and has a certain promoting effect on the clinical liver injury of Psoralea corylifolia L (Fructus Psoraleae). This study investigated the underlying mechanisms of psoralen-induced hepatotoxicity in vitro. HepG2 cells were treated with psoralen for 6, 12, 24, or 48 h, and an endoplasmic reticulum (ER) stress-specific inhibitor, 4-PBA, was employed to investigate the mechanism of psoralen on ER stress and unfolded protein response (UPR). Cell viability was tested by MTT assay, ATP assay, and cell death by LDH. The apoptosis was reflected by the flow cytometry, caspase-8, and caspase-3 activates. The expression of ER stress-related markers was determined by RT-PCR and western blot. We found that psoralen significantly decreased cell viability, increased activities of caspase-8 and caspase-3, and upregulated expression of CHOP and BAX in a time- and dose-dependent manner. Moreover, psoralen significantly increased the expression and transcription levels of ER stress-related markers, including Grp78, PERK, eIF2α, ATF4, and ATF6, while IRE1α was not significantly affected. And 4-PBA could effectively inhibit psoralen-induced cell death and apoptosis along with the inhibition of ER stress respons...Continue Reading
References
Dec 1, 1989·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·M KuboJ Iwamura
Sep 15, 2004·Journal of Chemical Neuroanatomy·Xiaohua ShenRandal J Kaufman
Sep 13, 2005·Surgery·Mario VilatobaJuan L Contreras
Feb 24, 2007·Planta medica·Chi-Rei WuHui Ching
Jan 13, 2009·PloS One·Jonathan H LinPeter Walter
Mar 6, 2012·Pharmacology & Therapeutics·Adrienne M GormanAfshin Samali
Jun 2, 2012·Cell Death and Differentiation·F AllagnatA K Cardozo
Dec 12, 2012·Scientific Reports·Yuhya WakasaFumio Takaiwa
Aug 31, 2013·Biochimica Et Biophysica Acta·Hery UrraClaudio Hetz
Oct 22, 2013·Journal of Molecular and Cellular Cardiology·Christopher C Glembotski
Nov 14, 2013·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Wei D LiZhi P Chen
Apr 23, 2014·Toxicological Sciences : an Official Journal of the Society of Toxicology·Lisa FredrikssonBob van de Water
Nov 13, 2014·The Biochemical Journal·Jianhua RaoFeng Zhang
Dec 5, 2014·Pharmaceutical Biology·Kang XuLi Yang
Jun 27, 2015·The Canadian Journal of Cardiology·Xi ZhaSidong Xiong
Aug 1, 2015·Cell Death & Disease·Z LiuJ Wang
Aug 11, 2015·Oncotarget·Yufeng WangKazuyuki Nakamura
Nov 8, 2015·Biological Trace Element Research·Syed Husain Mustafa RizviAbbas Ali Mahdi
Jan 23, 2016·The British Journal of Dermatology·P Wolf
Sep 2, 2016·PloS One·Mark OldhamNeil L Spector
Nov 5, 2016·The Journal of Biological Chemistry·Arvin Iracheta-VellveGyongyi Szabo
May 10, 2017·International Journal of Molecular Sciences·Andrea IorgaNeil Kaplowitz
Oct 11, 2017·Toxicology·Zhen RenLei Guo
Nov 17, 2017·World Journal of Gastroenterology : WJG·Fang-Wan YangShi-De Lin
Dec 2, 2017·Oncotarget·Bo RenYulin Li
Dec 16, 2017·Phytotherapy Research : PTR·Fiaz AlamMuhammad Hassham Hassan Bin Asad
Mar 15, 2018·Cell Reports·Justine LebeauR Luke Wiseman
Mar 20, 2018·PloS One·Eri SoejimaKentaro Yamada
Aug 23, 2018·Chemical Research in Toxicology·Yue ZhangKun Zhou
Jan 9, 2019·Frontiers in Pharmacology·Qing XiaKechun Liu
Jan 15, 2019·Journal of Ethnopharmacology·Jung-Hwan ByeonChang-Gue Son
Jan 23, 2019·Current Hepatology Reports·Paul H Hayashi, Einar S Bjornsson
Citations
Oct 6, 2020·Frontiers in Pharmacology·Yali RenCheng Peng
Dec 16, 2020·Human & Experimental Toxicology·Juyang HuangKun Zhou
Dec 14, 2019·Chinese Journal of Natural Medicines·Qi-Hang ZhouGuang-Bo Ge
Feb 10, 2021·Drug and Chemical Toxicology·Ruili YuKun Zhou
Mar 20, 2021·Journal of Molecular Neuroscience : MN·Liang HanYuxiu Shi
Apr 14, 2020·ACS Omega·Li YangKun Zhou