Psoriasin (S100A7) is a novel biomarker for lung squamous cell carcinoma in humans

Cancer Cell International
Guijuan LiuJihong Zhang

Abstract

Psoriasin (S100A7) plays a role in the malignant potential of several epithelial cancers, and could candidate diagnostic marker or therapeutic target. Nuclear factor kappa B (NF-κB) regulates cancer cell growth and is modulated by phospholipase activity in many cancer cells. In the present study, we first evaluate the involvement of S100A7 in lung squamous cell carcinoma and its clinical usefulness for diagnosis. We then study whether knockdown of S100A7 in lung squamous cell carcinoma cells would reduce cell proliferation and NF-κB activity in vitro and attenuate tumor growth in vivo. We examined S100A7 expression in lung squamous cell carcinoma tissues by immunohistology. The human lung squamous cell carcinoma cell line NCI-H520 were transduced with short hairpin RNA targeting S100A7. Quantitative reverse transcriptase-polymerase chain reaction and immunoblotting confirmed knockdown of S100A7 messenger RNA and protein, respectively. Cell proliferation was evaluated by the MTT assay. NF-κB phosphorylation was assayed by western blot. 1 × 10(6) of NCI-H520/S100A7 knockdown cells were injected into the left flanks of nude mice (aged 6 to 8 weeks). Tumors were followed for 35 days, then removed and stained with hematoxylin and eo...Continue Reading

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Citations

Jan 16, 2016·International Journal of Molecular Sciences·Roberta LottiCarlo Pincelli
Aug 31, 2018·Immunogenetics·Ana Águeda-Pinto, Pedro José Esteves
Nov 16, 2017·Cancers·Tatiana N ZamayAnna S Kichkailo
Jun 6, 2021·Clinica Chimica Acta; International Journal of Clinical Chemistry·Ting WangDong Wang
Jul 10, 2021·Pulmonary Medicine·Zeeshan SattarPatrick Geraghty

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Methods Mentioned

BETA
light microscopy
xenograft
PCR
electrophoresis
xenografts

Software Mentioned

SPSS
ImageJ
LightCycler

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